Anti-SOCS3抗体(ab53984)
Key features and details
- Rabbit polyclonal to SOCS3
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SOCS3抗体
参阅全部 SOCS3 一抗 -
描述
兔多克隆抗体to SOCS3 -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human SOCS3 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O14543 -
常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53984于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. 30 minutes at room temperature.
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| 说明 |
|---|
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IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. 30 minutes at room temperature. |
靶标
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功能
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. -
组织特异性
Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients. -
序列相似性
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
结构域
The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. -
翻译后修饰
Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1. - Information by UniProt
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数据库链接
- Entrez Gene: 9021 Human
- Omim: 604176 Human
- SwissProt: O14543 Human
- Unigene: 527973 Human
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别名
- ATOD4 antibody
- CIS 3 antibody
- CIS-3 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS3 antibody (ab53984)Ab53984 staining Human normal jejunum. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab53984 被引用在 6 文献中.
- Zhang M et al. High Inflammatory Factor Grading Predicts Poor Disease-Free Survival in AJCC Stage I-II Hepatocellular Carcinoma Patients After R0 Resection. Cancer Manag Res 11:10623-10632 (2019). PubMed: 31908534
- Gielen V et al. Increased nuclear suppressor of cytokine signaling 1 in asthmatic bronchial epithelium suppresses rhinovirus induction of innate interferons. J Allergy Clin Immunol 136:177-188.e11 (2015). PubMed: 25630941
- Walker DG et al. Expression of suppressor of cytokine signaling genes in human elderly and Alzheimer's disease brains and human microglia. Neuroscience 302:121-37 (2015). PubMed: 25286386
- Pascarella S et al. Intrahepatic mRNA levels of SOCS1 and SOCS3 are associated with cirrhosis but do not predict virological response to therapy in chronic hepatitis C. Liver Int 33:94-103 (2013). PubMed: 23164156
- White GE et al. Suppressor of cytokine signalling protein SOCS3 expression is increased at sites of acute and chronic inflammation. J Mol Histol 42:137-51 (2011). WB, ICC/IF ; Human . PubMed: 21360047
- Li Y et al. Disease-related expression of the IL6/STAT3/SOCS3 signalling pathway in ulcerative colitis and ulcerative colitis-related carcinogenesis. Gut 59:227-35 (2010). IHC-P ; Human . PubMed: 19926618