AB280895

重组Anti-SOCS1抗体[EPR24290-356] - BSA and Azide free

Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal SOCS1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 1 publication.

查看别名

SSI1, TIP3, SOCS1, Suppressor of cytokine signaling 1, SOCS-1, JAK-binding protein, STAT-induced STAT inhibitor 1, Tec-interacting protein 3, JAB, SSI-1, TIP-3

4 Images

Flow Cytometry (Intracellular) - Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (AB280895)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (AB280895)

This data was developed using ab280886, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized U266B1 (human multiple myeloma B lymphocyte, left)/ KARPAS-299 (Human anaplastic large cell lymphoma, right) cells labelling SOCS1 with ab280886 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.Negative control : U266B1 (PMID : 15580314).

Supplier Data

Immunoprecipitation - Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (AB280895)

This data was developed using ab280886, the same antibody clone in a different buffer formulation.

SOCS1 was immunoprecipitated from 0.35 mg of KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate with ab280886 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab280886 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate 10 ug

Lane 2 : ab280886 IP in KARPAS-299 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab280886 in mouse eyeball tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 93 seconds

All lanes:

Immunoprecipitation - Anti-SOCS1 antibody [EPR24290-356] (<a href='/products/primary-antibodies/socs1-antibody-epr24290-356-ab280886'>ab280886</a>)

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Lab

Western blot - Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (AB280895)

This data was developed using ab280886, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 15580314, 26090662, 10490100).

Negative control : U266B1 (PMID : 15580314).

Exposure time : Lane 1 : 48 seconds Lane 2-3 : 3 minutes

All lanes:

Western blot - Anti-SOCS1 antibody [EPR24290-356] (<a href='/products/primary-antibodies/socs1-antibody-epr24290-356-ab280886'>ab280886</a>) at 1/1000 dilution

Lane 1:

KARPAS-299 (human anaplastic large cell lymphoma) whole cell lysate at 60 µg

Lane 2:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 60 µg

Lane 3:

U266B1 (human multiple myeloma B lymphocyte) whole cell lysate at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 22 kDa,23 kDa

false

Lab

Western blot - Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (AB280895)

This data was developed using ab280886, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 15580314, 26090662, 10490100).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-SOCS1 antibody [EPR24290-356] (<a href='/products/primary-antibodies/socs1-antibody-epr24290-356-ab280886'>ab280886</a>) at 1/1000 dilution

All lanes:

Mouse thymus tissue lysate at 60 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR24290-356

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Human

应用

Flow Cyt (Intra), IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

ab280895 is the carrier-free version of ab280886.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Constituents: PBS

运输条件

Blue Ice

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Essential negative regulator of type I and type II interferon (IFN) signaling, as well as that of other cytokines, including IL2, IL4, IL6 and leukemia inhibitory factor (LIF) (PubMed : 32499645, PubMed : 33087723). Downregulates cytokine signaling by inhibiting the JAK/STAT signaling pathway. Acts by binding to JAK proteins and to IFNGR1 and inhibiting their kinase activity. In vitro, suppresses Tec protein-tyrosine activity (PubMed : 9341160). Regulates IFN-gamma (IFNG)-mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed : 11278610, PubMed : 11313480).

See full target information SOCS1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1548802 PubMed40534860

2025

Exosomal let-7b-5p derived from Aspergillus fumigatus-treated human corneal epithelial cells promotes M1 macrophage activation via targeting SOCS-1.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoming Yu,Xinyi Wu

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-SOCS1抗体[EPR24290-356] - BSA and Azide free

Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (AB280895)

Immunoprecipitation - Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (AB280895)

Western blot - Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (AB280895)

Western blot - Anti-SOCS1 antibody [EPR24290-356] - BSA and Azide free (AB280895)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原