Anti-SOCS1抗体(ab62584)
Key features and details
- Rabbit polyclonal to SOCS1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SOCS1抗体
参阅全部 SOCS1 一抗 -
描述
兔多克隆抗体to SOCS1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic 15 amino acid peptide from near the carboxy terminus of human SOCS1.
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阳性对照
- WB: Human spleen cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62584于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 24 kDa).
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| 说明 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 24 kDa). |
靶标
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功能
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2. -
组织特异性
Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes. -
通路
Protein modification; protein ubiquitination. -
序列相似性
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
结构域
The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box. - Information by UniProt
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数据库链接
- Entrez Gene: 8651 Human
- Omim: 603597 Human
- SwissProt: O15524 Human
- Unigene: 50640 Human
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别名
- CIS1 antibody
- CISH 1 antibody
- CISH1 antibody
see all
图片
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Western blot - Anti-SOCS1 antibody (ab62584)Lane 1 : Anti-SOCS1 antibody (ab62584) at 1 µg/ml
Lane 2 : Anti-SOCS1 antibody (ab62584) at 2 µg/ml
Lane 3 : Anti-SOCS1 antibody (ab62584) at 4 µg/ml
All lanes : Human spleen
cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 24 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (30)
ab62584 被引用在 30 文献中.
- Gong H et al. LncRNA KIAA0087 suppresses the progression of osteosarcoma by mediating the SOCS1/JAK2/STAT3 signaling pathway. Exp Mol Med 55:831-843 (2023). PubMed: 37009803
- Liang XS et al. IRAK-M Ablation Promotes Status Epilepticus-Induced Neuroinflammation via Activating M1 Microglia and Impairing Excitatory Synaptic Function. Mol Neurobiol 60:5199-5213 (2023). PubMed: 37277682
- Zhang Z et al. Macrophage‑derived exosomal miRNA‑155 promotes tubular injury in ischemia‑induced acute kidney injury. Int J Mol Med 50:N/A (2022). PubMed: 35795997
- Zhou C et al. Cancer-secreted exosomal miR-1468-5p promotes tumor immune escape via the immunosuppressive reprogramming of lymphatic vessels. Mol Ther 29:1512-1528 (2021). PubMed: 33388421
- Wu H et al. Mer regulates microglial/macrophage M1/M2 polarization and alleviates neuroinflammation following traumatic brain injury. J Neuroinflammation 18:2 (2021). PubMed: 33402181