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重组RabMAb

重组Anti-SNX5抗体[EPR14358] (ab180520)

Reviews (2) Submit a question References (11)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)
  • Western blot - Anti-SNX5 antibody [EPR14358] (ab180520)
  • Immunocytochemistry/ Immunofluorescence - Anti-SNX5 antibody [EPR14358] (ab180520)
  • Flow Cytometry - Anti-SNX5 antibody [EPR14358] (ab180520)
  • Western blot - Anti-SNX5 antibody [EPR14358] (ab180520)
  • Anti-SNX5 antibody [EPR14358] (ab180520)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14358] to SNX5
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-SNX5抗体[EPR14358]
    参阅全部 SNX5 一抗

  • 描述

    兔单克隆抗体[EPR14358] to SNX5

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, WB, IHC-P, Flow Cytmore details
    不适用于: IP

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • ICC/IF: 293T cell.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR14358

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab180520于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/50.

For unpurified use at 1/100.
WB (2)
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/50. 
Flow Cyt
1/50.
说明
ICC/IF
1/50.

For unpurified use at 1/100.
WB
1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/50. 
Flow Cyt
1/50.
应用说明
Is unsuitable for IP.

靶标

  • 功能

    May be involved in several stages of intracellular trafficking. Plays a role in macropinocytosis. Plays a role in the internalization of EGFR after EGF stimulation.

  • 序列相似性

    Belongs to the sorting nexin family.
    Contains 1 BAR domain.
    Contains 1 PX (phox homology) domain.

  • 结构域

    The PX domain mediates interaction with membranes enriched in phosphatidylinositol 3,4-bisphosphate and/or phosphatidylinositol 4,5-bisphosphate.

  • 细胞定位

    Early endosome membrane. Cell membrane. Cytoplasmic vesicle membrane. Cytoplasm. Cell projection > phagocytic cup. Cell projection > ruffle. Recruited to the plasma membrane after EGF stimulation, which leads to increased levels of phosphatidylinositol 3,4-bisphosphate (PdtIns(3,4)P2). Detected on macropinosomes. Targeted to membrane ruffles in response to EGFR stimulation.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • 0910001N05Rik antibody
    • 1810032P22Rik antibody
    • AU019504 antibody
    • D2Ertd52e antibody
    • FLJ10931 antibody
    • OTTHUMP00000030340 antibody
    • OTTHUMP00000062927 antibody
    • OTTMUSP00000003367 antibody
    • OTTMUSP00000003368 antibody
    • RP11-504H3.2 antibody
    • RP23-35E16.2 antibody
    • snoRNA MBI-43 antibody
    • SNX 5 antibody
    • SNX5 antibody
    • SNX5_HUMAN antibody
    • Sorting nexin 5 antibody
    • Sorting nexin-5 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labelling SNX5 with purified ab180520 at 1/500 dilution (0.99 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labelling SNX5 with purified ab180520 at 1/500 dilution (0.99 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX5 antibody [EPR14358] (ab180520)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue sections labelling SNX5 with purified ab180520 at 1/500 dilution (0.99 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-SNX5 antibody [EPR14358] (ab180520)
    Western blot - Anti-SNX5 antibody [EPR14358] (ab180520)
    All lanes : Anti-SNX5 antibody [EPR14358] (ab180520) at 1/1000 dilution (Purified)

    Lane 1 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
    Lane 2 : Mouse spleen lysate
    Lane 3 : C6 (Rat glial tumor glial cell) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SNX5 antibody [EPR14358] (ab180520)
    Immunocytochemistry/ Immunofluorescence - Anti-SNX5 antibody [EPR14358] (ab180520)

    Immunocytochemistry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling SNX5 with purified ab180520 at 1/50 dilution (9.9 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-SNX5 antibody [EPR14358] (ab180520)
    Flow Cytometry - Anti-SNX5 antibody [EPR14358] (ab180520)

    Flow cytometric analysis of 4% Paraformaldehyde fixed 90% Methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte) cells labelling SNX5 with ab180520 at 1/50 dilution (10 µg/ml) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

  • Western blot - Anti-SNX5 antibody [EPR14358] (ab180520)
    Western blot - Anti-SNX5 antibody [EPR14358] (ab180520)
    Anti-SNX5 antibody [EPR14358] (ab180520) at 1/1000 dilution (Purified) + Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Anti-SNX5 antibody [EPR14358] (ab180520)
    Anti-SNX5 antibody [EPR14358] (ab180520)

文献 (11)

发表研究结果有使用 ab180520？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab180520 被引用在 11 文献中.

  • Lopez-Robles C  et al. Architecture of the ESCPE-1 membrane coat. Nat Struct Mol Biol 30:958-969 (2023). PubMed: 37322239
  • Simonetti B  et al. ESCPE-1 mediates retrograde endosomal sorting of the SARS-CoV-2 host factor Neuropilin-1. Proc Natl Acad Sci U S A 119:e2201980119 (2022). PubMed: 35696571
  • Solinger JA  et al. FERARI and cargo adaptors coordinate cargo flow through sorting endosomes. Nat Commun 13:4620 (2022). PubMed: 35941155
  • Li Z Overexpression of lncRNA HOXA-AS2 promotes the progression of oral squamous cell carcinoma by mediating SNX5 expression. BMC Mol Cell Biol 23:59 (2022). PubMed: 36528556
  • Xu H  et al. SNX5 targets a monoamine transporter to the TGN for assembly into dense core vesicles by AP-3. J Cell Biol 221:N/A (2022). PubMed: 35426896
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-SNX5 antibody [EPR14358]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (kidney)
Gel Running Conditions
Reduced Denaturing (4-12% bis-tris gel, MES buffer)
Loading amount
30 µg
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 03 2020

Western blot abreview for Anti-SNX5 antibody [EPR14358]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Dopaminergic Neurons)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Dopaminergic Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 19 2020

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