Anti-SNX3抗体(ab56078)
Key features and details
- Rabbit polyclonal to SNX3
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
概述
-
产品名称
Anti-SNX3抗体
参阅全部 SNX3 一抗 -
描述
兔多克隆抗体to SNX3 -
宿主
Rabbit -
经测试应用
适用于: ELISA, WB, ICC/IFmore details -
种属反应性
与反应: Human, Recombinant fragment
预测可用于: Mouse
-
免疫原
Synthetic peptide (Human) sequence C-terminal end.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab56078于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein. |
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein. |
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
靶标
-
功能
Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol-3-phosphate (PtdIns(P3)). Plays a role in protein transport between cellular compartments. Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G (By similarity). Not involved in EGFR degradation. -
疾病相关
A chromosomal aberration involving SNX3 may be a cause of microphthalmia syndromic type 8 (MCOPS8) [MIM:601349]. Translocation t(6;13)(q21;q12). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS8 is a very rare congenital syndrome characterized by microcephaly, microphthalmia, ectrodactyly of the lower limbs and prognathism. Intellectual deficit has been reported. -
序列相似性
Belongs to the sorting nexin family.
Contains 1 PX (phox homology) domain. -
结构域
The PX domain mediates specific binding to phosphatidylinositol-3-phosphate (PtdIns(P3)). -
翻译后修饰
Ubiquitinated, leading to its proteasomal degradation. Deubiquitinated by USP10. -
细胞定位
Early endosome. - Information by UniProt
-
数据库链接
- Entrez Gene: 8724 Human
- Entrez Gene: 54198 Mouse
- Omim: 605930 Human
- SwissProt: O60493 Human
- SwissProt: O70492 Mouse
- Unigene: 12102 Human
- Unigene: 277870 Mouse
-
别名
- Grd19 antibody
- MCOPS8 antibody
- MGC151262 antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-SNX3 antibody (ab56078)ICC/IF image of ab56078 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56078, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
数据表及文件
-
SDS download
-
Datasheet download
文献 (8)
ab56078 被引用在 8 文献中.
- Cicek E et al. EGF-SNX3-EGFR axis drives tumor progression and metastasis in triple-negative breast cancers. Oncogene 41:220-232 (2022). PubMed: 34718348
- Yang Z et al. Multifaceted Roles of Retromer in EGFR Trafficking and Signaling Activation. Cells 11:N/A (2022). PubMed: 36359754
- Cui Y et al. Formation of retromer transport carriers is disrupted by the Parkinson disease-linked Vps35 D620N variant. Traffic 22:123-136 (2021). PubMed: 33347683
- Cui Y et al. Retromer has a selective function in cargo sorting via endosome transport carriers. J Cell Biol 218:615-631 (2019). PubMed: 30559172
- Pan B et al. SNX3 suppresses the migration and invasion of colorectal cancer cells by reversing epithelial-to-mesenchymal transition via the ß-catenin pathway. Oncol Lett 18:5332-5340 (2019). PubMed: 31612043
- Miller HE et al. Actin polymerization in the endosomal pathway, but not on the Coxiella-containing vacuole, is essential for pathogen growth. PLoS Pathog 14:e1007005 (2018). PubMed: 29668757
- Lucas M et al. Structural Mechanism for Cargo Recognition by the Retromer Complex. Cell 167:1623-1635.e14 (2016). PubMed: 27889239
- Morel E et al. Phosphatidylinositol-3-phosphate regulates sorting and processing of amyloid precursor protein through the endosomal system. Nat Commun 4:2250 (2013). PubMed: 23907271