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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-SNX1抗体(ab995)

  • Datasheet
  • SDS
Reviews (2)Q&A (3)References (9)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX1 antibody (ab995)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX1 antibody (ab995)

Key features and details

  • Rabbit polyclonal to SNX1
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-SNX1抗体
    参阅全部 SNX1 一抗
  • 描述

    兔多克隆抗体to SNX1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IHC-Pmore details
  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to Human SNX1 conjugated to keyhole limpet haemocyanin. Within exon 15 (LocusLink ID 6642)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab995于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能

    May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2).
  • 序列相似性

    Belongs to the sorting nexin family.
    Contains 1 BAR domain.
    Contains 1 PX (phox homology) domain.
  • 细胞定位

    Endosome membrane. Golgi apparatus > trans-Golgi network membrane. Early endosome membrane. Enriched on tubular elements of the early endosome membrane. Binds preferentially to highly curved membranes enriched in phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIn.
  • Target information above from: UniProt accession Q13596 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 427480 Chicken
    • Entrez Gene: 512029 Cow
    • Entrez Gene: 478336 Dog
    • Entrez Gene: 6642 Human
    • Entrez Gene: 56440 Mouse
    • Entrez Gene: 100174193 Orangutan
    • Entrez Gene: 84471 Rat
    • Omim: 601272 Human
    • SwissProt: Q05B62 Cow
    • SwissProt: Q13596 Human
    • SwissProt: Q9WV80 Mouse
    • SwissProt: Q5RFP8 Orangutan
    • SwissProt: Q99N27 Rat
    • Unigene: 188634 Human
    • Unigene: 271891 Mouse
    • Unigene: 144614 Rat
    see all
  • 别名

    • HsT17379 antibody
    • MGC8664 antibody
    • SNX 1 antibody
    • SNX 1a antibody
    • Snx1 antibody
    • SNX1_HUMAN antibody
    • SNX1A antibody
    • Sorting nexin 1 antibody
    • Sorting nexin 1A antibody
    • Sorting nexin-1 antibody
    • Vps5 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX1 antibody (ab995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX1 antibody (ab995)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SNX1 with ab995 at 1/1000 (1µg/ml). Detection: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX1 antibody (ab995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNX1 antibody (ab995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling SNX1 with ab995 at 1/400 (2.5µg/ml). A Red-fluorescent goat anti-rabbit IgG (1/100) was used as the secondary antibody.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (9)

发表研究结果有使用 ab995?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab995 被引用在 9 文献中.

  • van Olst L  et al. Meningeal inflammation in multiple sclerosis induces phenotypic changes in cortical microglia that differentially associate with neurodegeneration. Acta Neuropathol 141:881-899 (2021). PubMed: 33779783
  • Del Olmo T  et al. APEX2-mediated RAB proximity labeling identifies a role for RAB21 in clathrin-independent cargo sorting. EMBO Rep 20:N/A (2019). PubMed: 30610016
  • Simonetti B  et al. Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR. J Cell Biol 216:3695-3712 (2017). PubMed: 28935633
  • Duclos CM  et al. Caspase-mediated proteolysis of the sorting nexin 2 disrupts retromer assembly and potentiates Met/hepatocyte growth factor receptor signaling. Cell Death Discov 3:16100 (2017). Human . PubMed: 28179995
  • Yamauchi M  et al. Myeloid dendritic cells stimulated by thymic stromal lymphopoietin promote Th2 immune responses and the pathogenesis of oral lichen planus. PLoS One 12:e0173017 (2017). IHC . PubMed: 28278185
  • Huang L  et al. 15d-PGJ2 Reduced Microglia Activation and Alleviated Neurological Deficit of Ischemic Reperfusion in Diabetic Rat Model. Biomed Res Int 2015:864509 (2015). PubMed: 26844229
  • MacDonald E  et al. USP8 controls the trafficking and sorting of lysosomal enzymes. Traffic 15:879-88 (2014). PubMed: 24894536
  • Zhou Y  et al. Uric acid induces renal inflammation via activating tubular NF-?B signaling pathway. PLoS One 7:e39738 (2012). PubMed: 22761883
  • Nielsen MS  et al. Sorting by the cytoplasmic domain of the amyloid precursor protein binding receptor SorLA. Mol Cell Biol 27:6842-51 (2007). WB ; Human . PubMed: 17646382

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-SNX1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (day 1 embryos)
Loading amount
30 µg
Specification
day 1 embryos
Gel Running Conditions
Reduced Denaturing (4-12% SDS-PAGE gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 21 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-SNX1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 17 2007

Question

How were these antibodies tested?

Read More

Abcam community

Verified customer

Asked on Feb 22 2012

Answer

Thank you for your call today and for your questions about these SNX1 antibodies.
I've heard back from the lab about these antibodies, and unfortunately there are no images available to send, but I did gather some information about the testing.
Ab995 was tested in HeLa cells and the band in Western blot was seen at approximately 70kDa. Ab76072 was tested in immunostaining on mouse cortical cell culture (1 day old) and the WB was done using rat DRG lysate.
Based on this, I think that ab76072 would be much more suitable for your assay. I hope this information will be useful, but please let me know if you have any further questions and I'll be happy to help.

Read More

Abcam Scientific Support

回复于 Feb 22 2012

Question

I am interested in knowing the peptide sequence of SNX1 against which the antibody was raised. Thank you very much.

Read More

Abcam community

Verified customer

Asked on Jul 15 2004

Answer

Development of the antigen for antibody production is a confidential/proprietary process which we do not have authority (or knowledge) to detail at this time. Our apologies.

Read More

Abcam Scientific Support

回复于 Jul 16 2004

Question

I am planning to purchase your antibody product,SNX1(ab995) forimmunocytochemistry and western blot studies. I've looked through the paper which you said used your antibody was used for. However,Dr.Kurten et al said they raised the antibody at Bethyl Labs. Is your antibody the exact same thing as Dr.Kurten used in his study(means raised at Bethyl Lab), or you started from making the same oligo-peptide according to thier paper?

Read More

Abcam community

Verified customer

Asked on Oct 13 2003

Answer

We have recently updated our datasheets but an error in the code meant that some of our references were listed as specific rather than general. Although we are trying to clear this up it has been difficult. We thank you for bringing this one to our attention and I have adjusted the datasheet so that it now reads general reference. We are not aware of any references that cite this antibody but we always guarantee tested applications on the datasheet. Sorry for any inconvenience that this may have caused.

Read More

Asdf Edo

Abcam Scientific Support

回复于 Oct 13 2003

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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