Anti-SNRNP200抗体(ab176715)
Key features and details
- Rabbit polyclonal to SNRNP200
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-SNRNP200抗体
参阅全部 SNRNP200 一抗 -
描述
兔多克隆抗体to SNRNP200 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human SNRNP200 aa 400-450. The exact sequence is proprietary. NP_054733.2
Sequence:GEALAPRQVLDLEDLVFTQGSHFMANKRCQLPDGSFRRQRKGYEEVHVPA L
Database link: O75643 -
阳性对照
- WB: HeLa, 293T, Jurkat and NIH3T3 whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human colon carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7.0-8.0 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab176715 was affinity purified using an epitope specific to SNRNP200 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab176715于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000.
|
|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 245 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
|
| 说明 |
|---|
|
IHC-P
1/1000. |
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WB
1/2000 - 1/10000. Predicted molecular weight: 245 kDa. |
|
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Putative RNA helicase involved in the second step of RNA splicing. May promote one or more conformational changes in the dynamic network of RNA-RNA interactions in the spliceosome. Appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices. -
组织特异性
Widely expressed. -
疾病相关
Defects in SNRNP200 are the cause of retinitis pigmentosa type 33 (RP33) [MIM:610359]. It is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. -
序列相似性
Belongs to the helicase family. SKI2 subfamily.
Contains 2 helicase ATP-binding domains.
Contains 2 helicase C-terminal domains.
Contains 2 SEC63 domains. -
结构域
Composed of two similar domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 23020 Human
- Entrez Gene: 320632 Mouse
- Omim: 601664 Human
- SwissProt: O75643 Human
- SwissProt: Q6P4T2 Mouse
- Unigene: 246112 Human
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别名
- Activating signal cointegrator 1 complex subunit 3-like 1 antibody
- ASCC3L1 antibody
- BRR2 antibody
see all
图片
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Western blot - Anti-SNRNP200 antibody (ab176715)All lanes : Anti-SNRNP200 antibody (ab176715) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 245 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-SNRNP200 antibody (ab176715)SNRNP200 was immunoprecipitated from 1mg HeLa whole cell lysate using ab176715 at 6 μg/mg lysate (Lane 2), a rabbit anti-SNRNP200 antibody recognizing an upstream epitope (Lane 1) or control IgG (Lane 3). 20% of the Immunoprecipitate was loaded per lane and then immunobotted using ab176715 at 1.0 μg/ml.
Detection:
Chemiluminescence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNRNP200 antibody (ab176715)Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections using ab176715 at 1/1000 dilution (1 µg/mL).
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab176715 被引用在 1 文献中.
- Su X et al. Depletion of SNRNP200 inhibits the osteo-/dentinogenic differentiation and cell proliferation potential of stem cells from the apical papilla. BMC Dev Biol 20:22 (2020). PubMed: 33203369