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Anti-SNPH抗体(ab69992)

Submit a review Q&A (2)References (1)
Western blot - Anti-SNPH antibody (ab69992)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody (ab69992)
  • Immunocytochemistry/ Immunofluorescence - Anti-SNPH antibody (ab69992)

Key features and details

  • Rabbit polyclonal to SNPH
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-SNPH抗体
    参阅全部 SNPH 一抗

  • 描述

    兔多克隆抗体to SNPH

  • 宿主

    Rabbit

  • 经测试应用

    适用于: ICC/IF, IHC-P, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide within Human SNPH aa 80-130 (N terminal). The exact sequence is proprietary.

  • 阳性对照

    • Human brain tissue lysate

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab69992于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 58 kDa).
说明
ICC/IF
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 5 µg/ml.
WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 58 kDa).

靶标

图片

  • Western blot - Anti-SNPH antibody (ab69992)
    Western blot - Anti-SNPH antibody (ab69992)
    Lane 1 : Anti-SNPH antibody (ab69992) at 2 µg/ml
    Lane 2 : Anti-SNPH antibody (ab69992) at 4 µg/ml

    All lanes : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 58 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?
    Additional bands at: 115 kDa, 120 kDa, 57 kDa, 65 kDa, 70 kDa, 84 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody (ab69992)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNPH antibody (ab69992)

    ab69992 at 5 µg/ml staining SNPH in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunocytochemistry/ Immunofluorescence - Anti-SNPH antibody (ab69992)
    Immunocytochemistry/ Immunofluorescence - Anti-SNPH antibody (ab69992)
    Immunofluorescence of Syntaphilin in Human Brain cells using ab69992 at 20 ug/ml.

文献 (1)

发表研究结果有使用 ab69992？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab69992 被引用在 1 文献中.

  • Feng Y  et al. Dl-3-n-Butylphthalide Alleviates Demyelination and Improves Cognitive Function by Promoting Mitochondrial Dynamics in White Matter Lesions. Front Aging Neurosci 13:632374 (2021). PubMed: 33762923

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

Sorry no at the present moment it will be using it on paraffin fixed tissue slides with a fluorescent dye conjugated secondary antibody.
Thanks

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Answer

Thank you for your swift reply.
Unfortunately, as IHC-P is an already tested application, we cannot give you the AbTrial testing discount this time.
However, you'll have our Abpromise guarantee: Our policy is that we are happy to offer a refund, credit note or free of charge replacement when a product is not working in a successfully tested applications or species (and the product has been purchased in the last 180 days).
I hope this information is still helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Hi, I would like to test the following product in the indicated application and/or species noted below:
Anti-SNPH antibody (ab69992) for use in immunofluorescence.

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Answer

Thank you for contacting us.
Could you please confirm that you want to use this antibody on cell culture, not at tissue slides with a fluorescent dye conjugated antibody?
I am looking forward to receive your reply.

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