重组Anti-SNF2L抗体[EPR12191] (ab172483)
![Western blot - Anti-SNF2L antibody [EPR12191] (ab172483)](https://www.abcam.cn/ps/products/172/ab172483/Images/ab172483-481117-anti-snf2l-antibody-epr12191-western-blot-mouse-rat.jpg "Western blot - Anti-SNF2L antibody [EPR12191] (ab172483)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12191] to SNF2L
- Suitable for: IP, WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-SNF2L抗体[EPR12191]
参阅全部 SNF2L 一抗 -
描述
兔单克隆抗体[EPR12191] to SNF2L -
宿主
Rabbit -
特异性
Flow Cyt (Intra) and IP results based on Human reactivity.
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经测试应用
适用于: IP, WB, Flow Cyt (Intra)more details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: C6, RAW264,7, PC-12, NIH/3T3, HeLa, 293T, SH-SY5Y and SK-OV03 whole cell lysates. IP: RAW264.7 whole cell lysate. Flow Cyt: 293T cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR12191 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172483于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
1/30.
|
|
| WB |
1/1000. Predicted molecular weight: 123 kDa.
|
|
| Flow Cyt (Intra) |
1/10.
|
| 说明 |
|---|
|
IP
1/30. |
|
WB
1/1000. Predicted molecular weight: 123 kDa. |
|
Flow Cyt (Intra)
1/10. |
应用说明
Is unsuitable for ICC/IF or IHC-P.
靶标
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相关性
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. Two transcript variants encoding different isoforms have been found for this gene. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 6594 Human
- Entrez Gene: 93761 Mouse
- Entrez Gene: 317575 Rat
- Omim: 300012 Human
- SwissProt: P28370 Human
- SwissProt: Q6PGB8 Mouse
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别名
- ATP dependent helicase SMARCA1 antibody
- global transcription activator homologous sequence antibody
- ISWI antibody
see all
图片
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Western blot - Anti-SNF2L antibody [EPR12191] (ab172483)All lanes : Anti-SNF2L antibody [EPR12191] (ab172483) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor glial cell) whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 123 kDa
Observed band size: 123 kDa
Exposure time: 81 secondsBlocking buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-SNF2L antibody [EPR12191] (ab172483)](https://www.abcam.cn/ps/products/172/ab172483/Images/ab172483-194826-anti-snf2l-antibody-epr12191-western-blot.jpg)
Western blot - Anti-SNF2L antibody [EPR12191] (ab172483)All lanes : Anti-SNF2L antibody [EPR12191] (ab172483) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : SK-OV-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 123 kDa -
![Immunoprecipitation - Anti-SNF2L antibody [EPR12191] (ab172483)](https://www.abcam.cn/ps/products/172/ab172483/Images/ab172483-481118-anti-snf2l-antibody-epr12191-immunoprecipitation-mouse.jpg)
Immunoprecipitation - Anti-SNF2L antibody [EPR12191] (ab172483)Blocking buffer and concentration: 5% NFDM/TBST.
All lanes : Anti-SNF2L antibody [EPR12191] (ab172483) at 1/1000 dilution
Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 10 µg
Lane 2 : RAW264.7 whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab172483 in RAW264.7 whole cell lysate
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 123 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
![Flow Cytometry (Intracellular) - Anti-SNF2L antibody [EPR12191] (ab172483)](https://www.abcam.cn/ps/products/172/ab172483/Images/ab172483-194827-anti-snf2l-antibody-epr12191-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SNF2L antibody [EPR12191] (ab172483)Intracellular flow cytometric analysis of permeabilized 293Tcells labeling SNF2L with ab172483at 1/10 dilution (red)or a Rabbit IgG (negative) (green).
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![Anti-SNF2L antibody [EPR12191] (ab172483)](https://www.abcam.cn/ps/products/172/ab172483/Images/ab172483-3-benefits-of-recombinant-antibodies.png)
Anti-SNF2L antibody [EPR12191] (ab172483)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab172483 被引用在 1 文献中.
- Alvarez-Saavedra M et al. Snf2h-mediated chromatin organization and histone H1 dynamics govern cerebellar morphogenesis and neural maturation. Nat Commun 5:4181 (2014). ChIP . PubMed: 24946904