AB250702

重组Anti-SNF2H抗体[EPR7673(2)] - BSA and Azide free

Anti-SNF2H antibody [EPR7673(2)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SNF2H antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

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SNF2H, WCRF135, SMARCA5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 5, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5, Sucrose nonfermenting protein 2 homolog, hSNF2H

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-SNF2H antibody [EPR7673(2)] - BSA and Azide free (AB250702)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SNF2H antibody [EPR7673(2)] - BSA and Azide free (AB250702)

This data was developed using ab183730, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HeLa cells (fixative -20°C Acetone) labeling SNF2H with ab183730 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 488) secondary used at a 1/200 diution. Right panel counterstain with Dapi.

Supplier Data

Western blot - Anti-SNF2H antibody [EPR7673(2)] - BSA and Azide free (AB250702)

This data was developed using ab183730, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SNF2H antibody [EPR7673(2)] (<a href='/products/primary-antibodies/snf2h-antibody-epr76732-ab183730'>ab183730</a>) at 1/5000 dilution

Lane 1:

HeLa lysate at 10 µg

Lane 2:

MCF7 lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 122 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-SNF2H antibody [EPR7673(2)]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR7673(2)

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab250702 is the carrier-free version of ab183730.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SNF2H also known as SMARCA5 is an ATP-dependent chromatin remodeler with a molecular mass of approximately 122 kDa. This protein belongs to the SNF2 family and functions as a motor protein utilizing the energy from ATP hydrolysis to alter chromatin structure. SNF2H expresses widely across multiple tissues with higher expression levels in the brain and reproductive organs. Its mechanical role involves sliding or repositioning nucleosomes along DNA which influences accessibility for transcription replication and repair machinery.

Biological function summary

In coordinating chromatin dynamics and nucleosome architecture SNF2H plays an important part in the ISWI (Imitation Switch) chromatin remodeling complex. This complex modulates chromatin organization to facilitate histone modification and DNA accessibility impacting gene expression regulation. SNF2H directly affects DNA replication and repair processes contributing to the maintenance of genomic stability. The structural flexibility provided by SNF2H is essential for cellular processes where timely and regulated DNA access is necessary.

Pathways

Modulation of chromatin architecture by SNF2H is key to both transcriptional regulation and DNA repair pathways. It participates in the DNA damage response pathway facilitating recruitment and assembly of repair proteins at damage sites. SNF2H also involves itself in transcriptional control through associations with transcription factors and other chromatin contractors such as NURF (Nucleosome Remodeling Factor) complex components which include proteins like BPTF. These interactions permit precise control of gene activation or repression in response to cellular signals.

Dysfunction of SNF2H impacts chromatin remodeling fidelity contributing to conditions such as cancer and developmental anomalies. Aberrations in SNF2H expression or activity can lead to genomic instability influencing cancer progression through altered transcriptional programs and impaired DNA repair mechanisms. Additionally SNF2H links to ATRX syndrome a developmental disorder associated with mutations in the related ATRX protein highlighting its involvement in maintaining chromatin integrity essential for normal development.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity (PubMed : 12972596, PubMed : 28801535). Catalytic subunit of ISWI chromatin-remodeling complexes, which form ordered nucleosome arrays on chromatin and facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair; this may require intact histone H4 tails (PubMed : 10880450, PubMed : 12198550, PubMed : 12434153, PubMed : 12972596, PubMed : 23911928, PubMed : 28801535). Within the ISWI chromatin-remodeling complexes, slides edge- and center-positioned histone octamers away from their original location on the DNA template (PubMed : 28801535). Catalytic activity and histone octamer sliding propensity is regulated and determined by components of the ISWI chromatin-remodeling complexes (PubMed : 28801535). The BAZ1A/ACF1-, BAZ1B/WSTF-, BAZ2A/TIP5- and BAZ2B-containing ISWI chromatin-remodeling complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template in an ATP-dependent manner (PubMed : 14759371, PubMed : 15543136, PubMed : 28801535). The CECR2- and RSF1-containing ISWI chromatin-remodeling complexes do not have the ability to slide mononucleosomes to the center of a DNA template (PubMed : 28801535). Binds to core histones together with RSF1, and is required for the assembly of regular nucleosome arrays by the RSF-5 ISWI chromatin-remodeling complex (PubMed : 12972596). Involved in DNA replication and together with BAZ1A/ACF1 is required for replication of pericentric heterochromatin in S-phase (PubMed : 12434153). Probably plays a role in repression of RNA polymerase I dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter (By similarity). Essential component of the WICH-5 ISWI chromatin-remodeling complex (also called the WICH complex), a chromatin-remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure (PubMed : 11980720, PubMed : 15543136). The WICH-5 ISWI chromatin-remodeling complex regulates the transcription of various genes, has a role in RNA polymerase I transcription (By similarity). Within the B-WICH complex has a role in RNA polymerase III transcription (PubMed : 16603771). Mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes (By similarity). Essential component of NoRC-5 ISWI chromatin-remodeling complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing (By similarity).

See full target information SMARCA5

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重组Anti-SNF2H抗体[EPR7673(2)] - BSA and Azide free