Anti-SNF2H抗体(ab72499)
Key features and details
- Rabbit polyclonal to SNF2H
- Suitable for: ICC/IF, IHC-P, WB, IP, IHC-FrFl
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SNF2H抗体
参阅全部 SNF2H 一抗 -
描述
兔多克隆抗体to SNF2H -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WB, IP, IHC-FrFlmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to a region between residues 50 and 100 of human SNF2H (NP_003592.2)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab72499 was affinity purified using an epitope specific to SNF2H immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72499于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| IHC-FrFl |
Use at an assay dependent concentration. PubMed: 24946904
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 µg/ml. |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 122 kDa (predicted molecular weight: 122 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
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IHC-FrFl
Use at an assay dependent concentration. PubMed: 24946904 |
靶标
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功能
Helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity. Complexes containing SMARCA5 are capable of forming ordered nucleosome arrays on chromatin; this may require intact histone H4 tails. Also required for replication of pericentric heterochromatin in S-phase specifically in conjunction with BAZ1A. Probably plays a role in repression of polI dependent transcription of the rDNA locus, through the recruitment of the SIN3/HDAC1 corepressor complex to the rDNA promoter. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. -
组织特异性
Ubiquitously expressed. -
序列相似性
Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 2 SANT domains. -
发展阶段
Overexpressed in CD34-positive erythrocyte progenitor cells in acute myeloid leukemia. Down-regulation correlates with hematologic remission following chemotherapy. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8467 Human
- Entrez Gene: 93762 Mouse
- Entrez Gene: 307766 Rat
- Omim: 603375 Human
- SwissProt: O60264 Human
- SwissProt: Q91ZW3 Mouse
- Unigene: 558422 Human
- Unigene: 246803 Mouse
see all -
别名
- 4933427E24Rik antibody
- D030040M08Rik antibody
- D330027N15Rik antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-SNF2H antibody (ab72499)ICC/IF image of ab72499 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72499, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-SNF2H antibody (ab72499)All lanes : Anti-SNF2H antibody (ab72499) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 122 kDa
Observed band size: 122 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-SNF2H antibody (ab72499)Detection of SNF2H by Western Blot of Immunprecipitate.
ab72499 at 1µg/ml staining SNF2H in HeLa whole cell lysate immunoprecipitated using ab72499 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNF2H antibody (ab72499)IHC image of ab72499 staining in human breast cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72499, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab72499 被引用在 13 文献中.
- Bayona-Feliu A et al. The chromatin network helps prevent cancer-associated mutagenesis at transcription-replication conflicts. Nat Commun 14:6890 (2023). PubMed: 37898641
- Amatullah H et al. Epigenetic reader SP140 loss of function drives Crohn's disease due to uncontrolled macrophage topoisomerases. Cell 185:3232-3247.e18 (2022). PubMed: 35952671
- Goodwin LR et al. Impaired SNF2L Chromatin Remodeling Prolongs Accessibility at Promoters Enriched for Fos/Jun Binding Sites and Delays Granule Neuron Differentiation. Front Mol Neurosci 14:680280 (2021). PubMed: 34295220
- Alda-Catalinas C et al. A Single-Cell Transcriptomics CRISPR-Activation Screen Identifies Epigenetic Regulators of the Zygotic Genome Activation Program. Cell Syst 11:25-41.e9 (2020). PubMed: 32634384
- Alvarez-Saavedra M et al. Snf2h Drives Chromatin Remodeling to Prime Upper Layer Cortical Neuron Development. Front Mol Neurosci 12:243 (2019). PubMed: 31680852