Anti-SND1 antibody [EPR28903-23]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] (AB322053)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining in human skeletal muscle.
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] (AB322053)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human cerebrum (PMID : 30214229).
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] (AB322053)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human colon carcinoma (PMID : 25965817).
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] (AB322053)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human colon.
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] (AB322053)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human hepatocellular carcinoma (PMID : 30321081).
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] (AB322053)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining in mouse skeletal muscle.
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] (AB322053)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse cerebrum.
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] (AB322053)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse colon.
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody [EPR28903-23] (AB322053)

Immunohistochemical analysis of paraffin-embedded Mouse liver tumor tissue labeling SND1 with ab322053 at 1/2000 (0.262 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse liver tumor.
The section was incubated with ab322053 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-SND1 antibody [EPR28903-23] (AB322053)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody was tested on frozen and fresh NIH/3T3 whole cell lysates and it was concluded that SND1 is the easy target for degradation.

To minimize protein degradation, cells (lane6-9) were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The band(around 60 kd) beneath the target band is likely to be degraded target fragment.

The band be lower MW band around 50 kDa is supposed to be non-specific binding.

All lanes:

Western blot - Anti-SND1 antibody [EPR28903-23] (ab322053) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

Lane 3:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Lanes 5 and 8:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 6:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 7:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 9:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 101 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-SND1 antibody [EPR28903-23] (AB322053)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the lower MW band around 50 kDa is supposed to be non-specific binding.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SND1 antibody [EPR28903-23] (ab322053) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting SND1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 101 kDa,36 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-SND1 antibody [EPR28903-23] (AB322053)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : skeletal muscle

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SND1 antibody [EPR28903-23] (ab322053) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse pancreas tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 20 µg

Lane 5:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 101 kDa,36 kDa

false

Exposure time: 6s

• I-ELISA

Supplier Data

Indirect ELISA - Anti-SND1 antibody [EPR28903-23] (AB322053)

Indirect ELISA analysis of ab322053 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Human SND1.

Antigen concentration : 1000 ng/ml