Anti-SND1抗体(ab223770)
Key features and details
- Rabbit polyclonal to SND1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SND1抗体
参阅全部 SND1 一抗 -
描述
兔多克隆抗体to SND1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Orangutan
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免疫原
Recombinant fragment corresponding to Human SND1 aa 750 to the C-terminus.
Database link: Q7KZF4 -
阳性对照
- IHC-P: Human pancreas, placenta, stomach and cerebral cortex tissue. WB: SK-BR-3, U-87 MG and EFO-21 cell lysates. ICC/IF: A431 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223770于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 102 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 102 kDa. |
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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功能
Functions as a bridging factor between STAT6 and the basal transcription factor. Plays a role in PIM1 regulation of MYB activity. Functions as a transcriptional coactivator for the Epstein-Barr virus nuclear antigen 2 (EBNA2). -
组织特异性
Ubiquitously expressed. -
序列相似性
Contains 4 TNase-like domains.
Contains 1 Tudor domain. -
翻译后修饰
Phosphorylated by PIM1 in vitro. -
细胞定位
Cytoplasm. Nucleus. Melanosome. In IL-4 stimulated cells colocalizes with STAT6 in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 404098 Cow
- Entrez Gene: 27044 Human
- Entrez Gene: 56463 Mouse
- Entrez Gene: 100189909 Orangutan
- Entrez Gene: 64635 Rat
- Omim: 602181 Human
- SwissProt: Q863B3 Cow
- SwissProt: Q7KZF4 Human
see all -
别名
- 100 kDa coactivator antibody
- EBNA 2 co activator (100kD) antibody
- EBNA2 coactivator p100 antibody
see all
图片
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Western blot - Anti-SND1 antibody (ab223770)Anti-SND1 antibody (ab223770) at 0.4 µg/ml + SK-BR-3 cell lysate
Predicted band size: 102 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody (ab223770)Immunohistochemical analysis of human cerebral cortex tissue labeling SND1 with ab223770 at 1/200 dilution. -
Western blot - Anti-SND1 antibody (ab223770)All lanes : Anti-SND1 antibody (ab223770) at 0.4 µg/ml
Lane 1 : U-87 MG cell lysate
Lane 2 : EFO-21 cell lysate
Predicted band size: 102 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody (ab223770)Immunohistochemical analysis of human stomach tissue labeling SND1 with ab223770 at 1/200 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody (ab223770)Immunohistochemical analysis of human placenta tissue labeling SND1 with ab223770 at 1/200 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody (ab223770)Immunohistochemical analysis of human pancreas tissue labeling SND1 with ab223770 at 1/200 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SND1 antibody (ab223770)Immunohistochemical analysis of human skeletal muscle tissue labeling SND1 with ab223770 at 1/200 dilution. No staining is observed.
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Immunocytochemistry/ Immunofluorescence - Anti-SND1 antibody (ab223770)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for SND1 (green) using ab223770 at 4 µg/ml in ICC/IF.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab223770 被引用在 1 文献中.
- Yao X et al. Protective effects of SND1 in retinal photoreceptor cell damage induced by ionizing radiation. Biochem Biophys Res Commun 514:919-925 (2019). PubMed: 31084926