Name: Anti-SNAP29 antibody [EPR9199]
Brand: RabMAb
SKU: ab138500
Rating: 5 (4 reviews)

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Western blot - Anti-SNAP29 antibody [EPR9199] (ab138500)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9199] to SNAP29
Suitable for: WB, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

产品名称

Anti-SNAP29抗体[EPR9199]
参阅全部 SNAP29 一抗
描述

兔单克隆抗体[EPR9199] to SNAP29
宿主

Rabbit
经测试应用

适用于: WB, IPmore details
不适用于: ICC/IF or IHC-P
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide within Human SNAP29 aa 200-300 (C terminal). The exact sequence is proprietary.
阳性对照
- WB: 293T, Jurkat, HepG2, and HeLa cell lysates.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
解离常数（K_D）

K_D = 1.30 x 10 ^-12 M
Learn more about K_D
存储溶液

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR9199
同种型

IgG
研究领域

Alternative Versions
- Anti-SNAP29 antibody [EPR9199] - BSA and Azide free (ab240110)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human SNAP29 knockout HeLa cell line (ab265289)
KO cell lysates
- Human SNAP29 knockout HeLa cell lysate (ab257693)
Positive Controls
- HeLa whole cell lysate (ab150035)
- Jurkat whole cell lysate (ab7899)

The Abpromise guarantee

Abpromise™承诺保证使用ab138500于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(4)	1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
IP		1/10 - 1/100.

说明
WB 1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
IP 1/10 - 1/100.

应用说明

Is unsuitable for ICC/IF or IHC-P.

功能

Involved in multiple membrane trafficking steps.
组织特异性

Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.
疾病相关

Defects in SNAP29 are the cause of CEDNIK syndrome (CEDNIK) [MIM:609528]. CEDNIK is a neurocutaneous syndrome characterized by cerebral dysgenesis, neuropathy, ichthyosis and palmoplantar keratoderma.
序列相似性

Belongs to the SNAP-25 family.
Contains 1 t-SNARE coiled-coil homology domain.
细胞定位

Cytoplasm. Membrane. Cell junction > synapse > synaptosome. Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic.
Target information above from: UniProt accession O95721 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 9342 Human
- Entrez Gene: 67474 Mouse
- Entrez Gene: 116500 Rat
- Omim: 604202 Human
- SwissProt: O95721 Human
- SwissProt: Q9ERB0 Mouse
- SwissProt: Q9Z2P6 Rat
- Unigene: 108002 Human
- Unigene: 701035 Human
- Unigene: 271992 Mouse
- Unigene: 17902 Rat
see all
别名
- CEDNIK antibody
- FLJ21051 antibody
- SNAP 29 antibody
- SNAP-29 antibody
- SNAP29 antibody
- SNP29_HUMAN antibody
- Soluble 29 kDa NSF attachment protein antibody
- Synaptosomal associated protein 29 antibody
- Synaptosomal associated protein 29kDa antibody
- Synaptosomal-associated protein 29 antibody
- Vesicle membrane fusion protein SNAP 29 antibody
- Vesicle membrane fusion protein SNAP29 antibody
- Vesicle-membrane fusion protein SNAP-29 antibody
see all

Western blot - Anti-SNAP29 antibody [EPR9199] (ab138500)

All lanes : Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SNAP29 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?

Lanes 1-3: Merged signal (red and green). Green - ab138500 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.

ab138500 Anti-SNAP29 antibody [EPR9199] was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used. Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE. ab138500 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-SNAP29 antibody [EPR9199] (ab138500)

All lanes : Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/2000 dilution (purified)

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-SNAP29 antibody [EPR9199] (ab138500)

All lanes : Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/2000 dilution (purified)

Lane 1 : HeLa cell lysate
Lane 2 : HEK293 cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunoprecipitation - Anti-SNAP29 antibody [EPR9199] (ab138500)

ab138500 (purified) at 1/20 immunoprecipitating SNAP29 in 10 μg HeLa cell lysate (Lanes 1 and 2, observed at 29 kDa). Lane 3 - Rabbit monoclonal IgG (ab172730). For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
Western blot - Anti-SNAP29 antibody [EPR9199] (ab138500)

All lanes : Anti-SNAP29 antibody [EPR9199] (ab138500) at 1/1000 dilution (unpurified)

Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
OI-RD Scanning - Anti-SNAP29 antibody [EPR9199] (ab138500)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-SNAP29 antibody [EPR9199] (ab138500)

Western blot protocols
Immunoprecipitation protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab138500？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab138500 被引用在 20 文献中.

Hessvik NP et al. siRNA screening reveals that SNAP29 contributes to exosome release. Cell Mol Life Sci 80:177 (2023). PubMed: 37285022
Jassey A et al. Starvation after infection restricts enterovirus D68 replication. Autophagy 19:112-125 (2023). PubMed: 35446171
Solvik TA et al. Secretory autophagy maintains proteostasis upon lysosome inhibition. J Cell Biol 221:N/A (2022). PubMed: 35446347
Miao G et al. ORF3a of the COVID-19 virus SARS-CoV-2 blocks HOPS complex-mediated assembly of the SNARE complex required for autolysosome formation. Dev Cell 56:427-442.e5 (2021). PubMed: 33422265
Cardenas RA et al. SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis. J Biol Chem 296:100268 (2021). PubMed: 33837726

View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A

提交评价提交问题

1-4 of 4 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Dopaminergic Neurons)

Gel Running Conditions

Reduced Denaturing (NuPage 4-12% MES buffer)

Loading amount

30 µg

Specification

Dopaminergic Neurons

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 22 2020

Application

Western blot

Sample

Mouse Cell lysate - other (Heart)

Gel Running Conditions

Reduced Denaturing (4-12%)

Loading amount

10 µg

Treatment

CRE virus knockdown

Specification

Heart

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Mar 09 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK293T, HCT116)

Gel Running Conditions

Reduced Denaturing

Loading amount

30 µg

Specification

HEK293T, HCT116

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Sep 06 2019

Application

Western blot

Loading amount

15 µg

Gel Running Conditions

Reduced Denaturing (12)

Sample

Mouse Tissue lysate - whole (Heart)

Specification

Heart

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 21 2013

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

解离常数（KD）

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Positive Controls

应用

The Abpromise guarantee

靶标

功能

组织特异性

疾病相关

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (20)

客户评价及客户问答

Western blot abreview for Anti-SNAP29 antibody [EPR9199]

Western blot abreview for Anti-SNAP29 antibody [EPR9199]

Western blot abreview for Anti-SNAP29 antibody [EPR9199]

Western blot

解离常数（K_D）