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使用敲除细胞株进行验证重组RabMAb

重组Anti-SNAP29抗体[EPR9198(2)] (ab181151)

Reviews (1) Submit a question References (8)
Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
  • Flow Cytometry (Intracellular) - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
  • Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
  • Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
  • Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9198(2)] to SNAP29
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-SNAP29抗体[EPR9198(2)]
    参阅全部 SNAP29 一抗

  • 描述

    兔单克隆抗体[EPR9198(2)] to SNAP29

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, ICC/IF, IPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HeLa, HepG2, 293 and Jurkat whole cell lysates. ICC/IF: HeLa cells. Flow Cyt (intra): Jurkat cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab181151于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ICC/IF
1/100 - 1/250.
IP
1/30 - 1/50.
说明
Flow Cyt (Intra)
1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
ICC/IF
1/100 - 1/250.
IP
1/30 - 1/50.

靶标

  • 功能

    Involved in multiple membrane trafficking steps.

  • 组织特异性

    Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.

  • 疾病相关

    Defects in SNAP29 are the cause of CEDNIK syndrome (CEDNIK) [MIM:609528]. CEDNIK is a neurocutaneous syndrome characterized by cerebral dysgenesis, neuropathy, ichthyosis and palmoplantar keratoderma.

  • 序列相似性

    Belongs to the SNAP-25 family.
    Contains 1 t-SNARE coiled-coil homology domain.

  • 细胞定位

    Cytoplasm. Membrane. Cell junction > synapse > synaptosome. Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • CEDNIK antibody
    • FLJ21051 antibody
    • SNAP 29 antibody
    • SNAP-29 antibody
    • SNAP29 antibody
    • SNP29_HUMAN antibody
    • Soluble 29 kDa NSF attachment protein antibody
    • Synaptosomal associated protein 29 antibody
    • Synaptosomal associated protein 29kDa antibody
    • Synaptosomal-associated protein 29 antibody
    • Vesicle membrane fusion protein SNAP 29 antibody
    • Vesicle membrane fusion protein SNAP29 antibody
    • Vesicle-membrane fusion protein SNAP-29 antibody
    see all

图片

  • Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    All lanes : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SNAP29 knockout HeLa cell lysate
    Lane 3 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab181151 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab181151 Anti-SNAP29 antibody [EPR9198(2)] was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used.  Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE.  ab181151 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

    Immunofluorescent analysis of -20°C acetone fixed Hela cells staining SNAP29 using ab181151 at 1/250 dilution and Alexa Fluor® 555 stained Goat anti Rabbit IgG at 1/250 as a secondary antibody (red). Dapi counterstain (blue).

  • Flow Cytometry (Intracellular) - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Flow Cytometry (Intracellular) - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

    Intracellular flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells staining SNAP29 using ab181151 at 1/10 dilution, and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve). Rabbit monoclonal IgG was used as the negative control (green curve). 

     

     

  • Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    All lanes : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/5000 dilution

    Lane 1 : HeLa cell lysate with NFDM/TBST
    Lane 2 : 293 cell lysate with NFDM/TBST
    Lane 3 : Jurkat cell lysate with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa

  • Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

    Western blot analysis of SNAP29 in immunoprecipitation pellets from Jurkat cell lysate, using ab181151 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as a secondary antibody at 1/1000 dilution. Blocking/dilution buffer and concentration: 5% NFDM/TBST



    Lane 1 : Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/50 dilution


    Lane 1 : Precipitate from Jurkat lysate using ab181151 with NFDM/TBST
    Lane 2 : Jurkat lysate with NFDM/TBST

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution
  • Anti-SNAP29 antibody [EPR9198(2)] (ab181151)
    Anti-SNAP29 antibody [EPR9198(2)] (ab181151)

文献 (8)

发表研究结果有使用 ab181151？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab181151 被引用在 8 文献中.

  • Sánchez-Martín P  et al. ULK1-mediated phosphorylation regulates the conserved role of YKT6 in autophagy. J Cell Sci 136:N/A (2023). PubMed: 36644903
  • Wang L  et al. Puerarin Prevents Cadmium-Induced Neuronal Injury by Alleviating Autophagic Dysfunction in Rat Cerebral Cortical Neurons. Int J Mol Sci 24:N/A (2023). PubMed: 37176033
  • Buchacher T  et al. Persistent coxsackievirus B1 infection triggers extensive changes in the transcriptome of human pancreatic ductal cells. iScience 25:103653 (2022). PubMed: 35024587
  • Yan W  et al. Decreased neuronal synaptosome associated protein 29 contributes to poststroke cognitive impairment by disrupting presynaptic maintenance. Theranostics 11:4616-4636 (2021). PubMed: 33754017
  • Wu W  et al. TNF-induced necroptosis initiates early autophagy events via RIPK3-dependent AMPK activation, but inhibits late autophagy. Autophagy N/A:1-18 (2021). PubMed: 33779513
  • Xiang P  et al. SNAP47 Interacts with ATG14 to Promote VP1 Conjugation and CVB3 Propagation. Cells 10:N/A (2021). PubMed: 34440910
  • Chakraborty A  et al. SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network. J Cell Sci 133:N/A (2020). PubMed: 32184263
  • Huang L  et al. O-GlcNAc-modified SNAP29 inhibits autophagy-mediated degradation via the disturbed SNAP29-STX17-VAMP8 complex and exacerbates myocardial injury in type I diabetic rats. Int J Mol Med 42:3278-3290 (2018). PubMed: 30221662

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Western blot abreview for Anti-SNAP29 antibody [EPR9198(2)]

 Excellent
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Dec 04 2021

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