AB181151

重组Anti-SNAP29抗体[EPR9198(2)]

Name: SNAP29抗体[EPR9198(2)] (ab181151)| Abcam中文官网
Brand: Abcam Limited
SKU: AB181151
Price: 2282 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-SNAP29 antibody [EPR9198(2)]

RabMAb
Recombinant
KO Validated
了解详情

(1 Review)

(13 Publications)

Rabbit Recombinant Monoclonal SNAP29 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 13 publications.

查看别名

Synaptosomal-associated protein 29, SNAP-29, Soluble 29 kDa NSF attachment protein, Vesicle-membrane fusion protein SNAP-29, SNAP29

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] (AB181151)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SNAP29 antibody [EPR9198(2)] (AB181151)

Immunofluorescent analysis of -20°C acetone fixed Hela cells staining SNAP29 using ab181151 at 1/250 dilution and Alexa Fluor^® 555 stained Goat anti Rabbit IgG at 1/250 as a secondary antibody (red). Dapi counterstain (blue).

Flow Cytometry (Intracellular) - Anti-SNAP29 antibody [EPR9198(2)] (AB181151)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SNAP29 antibody [EPR9198(2)] (AB181151)

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed Jurkat cells staining SNAP29 using ab181151 at 1/10 dilution, and FITC conjugated Goat anti rabbit IgG at 1/150 as a secondary antibody (red curve). Rabbit monoclonal IgG was used as the negative control (green curve).

Supplier Data

Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] (AB181151)

Western blot analysis of SNAP29 in immunoprecipitation pellets from Jurkat cell lysate, using ab181151 at a 1/50 dilution.

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as a secondary antibody at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/50 dilution

Lane 1:

Precipitate from Jurkat lysate using ab181151

Lane 2:

Jurkat lysate

Secondary

All lanes:

Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 29 kDa

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Lab

Western blot - Anti-SNAP29 antibody [EPR9198(2)] (AB181151)

Lanes 1-3 : Merged signal (red and green). Green - ab181151 observed at 29 kDa. Red - loading control ab8245 observed at 36 kDa.

ab181151 Anti-SNAP29 antibody [EPR9198(2)] was shown to specifically react with SNAP29 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265289 (knockout cell lysate ab257693) was used. Wild-type and SNAP29 knockout samples were subjected to SDS-PAGE. ab181151 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

SNAP29 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human SNAP29 knockout HeLa cell line (<a href='/products/cell-lines/human-snap29-knockout-hela-cell-line-ab265289'>ab265289</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

Supplier Data

Western blot - Anti-SNAP29 antibody [EPR9198(2)] (AB181151)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SNAP29 antibody [EPR9198(2)] (ab181151) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Peroxidase conjugated Goat anti-Rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-SNAP29 antibody [EPR9198(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR9198(2)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, IP, WB, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SNAP29 known also as synaptosomal-associated protein 29 functions mainly in membrane fusion. It weighs about 29 kDa. This protein plays a significant role in intracellular membrane trafficking guiding vesicle fusion events. It is expressed in many tissues with high occurrence in neuronal and non-neuronal cells. SNAP29 contains coiled-coil domains that facilitate its role in binding with SNARE complexes which assist in the docking and fusion of vesicles with target membranes.

Biological function summary

RNA encoding this protein facilitates vesicle-mediated transport on the cellular level especially under conditions requiring rapid membrane addition or reshaping. SNAP29 acts alongside other SNARE proteins like syntaxin and VAMP in the SNARE complex to modulate membrane interactions. The protein eases the process of cytoplasmic content exchange between organelles. As part of these complexes SNAP29 maintains cellular homeostasis by contributing to membrane dynamics and trafficking processes.

Pathways

Vesicular transport in the cell highly depends on SNAP29 especially in exocytosis and endocytosis pathways. Alongside other SNARE proteins SNAP29 fits into the vesicle docking and fusion pathways ensuring efficient membrane recycling and cargo delivery. It interacts with several proteins in the process including syntaxin 1 and VAMP2 to facilitate these important functions within these pathways.

Impairments or mutations in the SNAP29 gene link to conditions like CEDNIK syndrome and other neurodevelopmental disorders. CEDNIK syndrome arises from disrupted membrane trafficking and organelle biogenesis due to SNAP29 dysfunction. In these cases a relationship with other proteins in the disease pathways such as STXBP1 can also alter disease progression and severity influencing neuronal communication and stability.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also plays a role in ciliogenesis by regulating membrane fusions.

See full target information Synaptosomal-associated protein 29

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of extracellular vesicles 13:e70002 PubMed39497621

2024

A switch from lysosomal degradation to secretory autophagy initiates osteogenic bone metastasis in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Wei,Mengmeng Liang,Min Deng,Ji Zheng,Fei Luo,Qinyu Ma

Traffic (Copenhagen, Denmark) 24:355-379 PubMed37340984

2023

Syntaxin-5's flexibility in SNARE pairing supports Golgi functions.

Applications

Unspecified application

Species

Unspecified reactive species

Zinia D'Souza,Irina Pokrovskaya,Vladimir V Lupashin

International journal of molecular sciences 24: PubMed37176033

2023

Puerarin Prevents Cadmium-Induced Neuronal Injury by Alleviating Autophagic Dysfunction in Rat Cerebral Cortical Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Tao Wang,Shuangquan Wen,Ruilong Song,Hui Zou,Jianhong Gu,Xuezhong Liu,Jianchun Bian,Zongping Liu,Yan Yuan

Acta biochimica et biophysica Sinica 54:1822-1831 PubMed36789686

2023

ox-LDL induces autophagy-mediated apoptosis by suppressing secretagogin-regulated autophagic flux in pancreatic β-cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Lv,Sijie Xiao,Shuhui Ouyang,Zhengliang Peng,Li Wu,Ziqing Tang,Weizheng Zhang,Renxian Cao,Jing Yang

Journal of cell science 136: PubMed36644903

2023

ULK1-mediated phosphorylation regulates the conserved role of YKT6 in autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Pablo Sánchez-Martín,Franziska Kriegenburg,Ludovico Alves,Julius Adam,Jana Elsaesser,Riccardo Babic,Hector Mancilla,Mariya Licheva,Georg Tascher,Christian Münch,Stefan Eimer,Claudine Kraft

Cell death discovery 8:435 PubMed36316321

2022

CUR5g, a novel autophagy inhibitor, exhibits potent synergistic anticancer effects with cisplatin against non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jingxuan Chen,Yunpeng Shen,Bowen Wu,Peichang Yang,Gangchun Sun,Xiaoting Liu,Pengfei Qiang,Yamei Gao,Fangfang Sha,Zirui Li,Lu Zhang

Journal of inherited metabolic disease 45:1191-1202 PubMed36102069

2022

Aberrant autophagy in lysosomal storage disorders marked by a lysosomal SNARE protein shortage due to suppression of endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Tanaka,Daisuke Tsuji,Ryosuke Watanabe,Yukiya Ohnishi,Shindai Kitaguchi,Ryuto Nakae,Hiromi Teramoto,Jun Tsukimoto,Yuto Horii,Kohji Itoh

iScience 25:103653 PubMed35024587

2022

Persistent coxsackievirus B1 infection triggers extensive changes in the transcriptome of human pancreatic ductal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Tanja Buchacher,Anni Honkimaa,Tommi Välikangas,Niina Lietzén,M Karoliina Hirvonen,Jutta E Laiho,Amir-Babak Sioofy-Khojine,Eeva-Liisa Eskelinen,Heikki Hyöty,Laura L Elo,Riitta Lahesmaa

Cells 10: PubMed34440910

2021

SNAP47 Interacts with ATG14 to Promote VP1 Conjugation and CVB3 Propagation.

Applications

Unspecified application

Species

Unspecified reactive species

Pinhao Xiang,Yasir Mohamud,Honglin Luo

Autophagy 17:3992-4009 PubMed33779513

2021

TNF-induced necroptosis initiates early autophagy events via RIPK3-dependent AMPK activation, but inhibits late autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxian Wu,Xiaojing Wang,Yadong Sun,Niklas Berleth,Jana Deitersen,David Schlütermann,Fabian Stuhldreier,Nora Wallot-Hieke,María José Mendiburo,Jan Cox,Christoph Peter,Ann Kathrin Bergmann,Björn Stork

View all publications

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重组Anti-SNAP29抗体[EPR9198(2)]