重组Anti-SNAP25抗体[EP3274] - BSA and Azide free (ab226046)
![Western blot - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)](https://www.abcam.cn/ps/products/226/ab226046/Images/ab226046-473216-anti-snap25-antibody-ep3274-bsa-and-azide-free-western-blot-wildtype-shsy5y-snap25-knockout-shsy5y-neuro2a-k562.jpg "Western blot - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3274] to SNAP25 - BSA and Azide free
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-SNAP25抗体[EP3274] - BSA and Azide free
参阅全部 SNAP25 一抗 -
描述
兔单克隆抗体[EP3274] to SNAP25 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: SH-SY5Y and Neuro-2a cell lysate.
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常规说明
ab226046 is the carrier-free version of ab108990.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP3274 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226046于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
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| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
|
WB
Use at an assay dependent concentration. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa). |
靶标
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功能
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. -
组织特异性
Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. -
序列相似性
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
翻译后修饰
Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. -
细胞定位
Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 6616 Human
- Entrez Gene: 20614 Mouse
- Entrez Gene: 25012 Rat
- Omim: 600322 Human
- SwissProt: P60880 Human
- SwissProt: P60879 Mouse
- SwissProt: P60881 Rat
- Unigene: 167317 Human
see all -
别名
- bA416N4.2 antibody
- Bdr antibody
- CMS18 antibody
see all
图片
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Western blot - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)All lanes : Anti-SNAP25 antibody [EP3274] (ab108990) at 1/1000 dilution
Lane 1 : Wild-type SH-SY5Y cell lysate
Lane 2 : SNAP25 knockout SH-SY5Y cell lysate
Lane 3 : Neuro-2a cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-SNAP25 antibody [EP3274] staining at 1/1000 dilution, shown in green; loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, ab108990 was shown to bind specifically to SNAP25. A band was observed at 27 kDa in wild-type SH-SY5Y cell lysates with no signal observed at this size in SNAP25 knockout cell line ab280041 (knockout cell lysate ab280100). To generate this image, wild-type and SNAP25 knockout SH-SY5Y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)](https://www.abcam.cn/ps/products/226/ab226046/Images/ab226046-322764-anti-snap25-antibody-ep3274-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human brain tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108990).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)](https://www.abcam.cn/ps/products/226/ab226046/Images/ab226046-322763-anti-snap25-antibody-ep3274-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)ab108990, at 1/100 dilution, staining SNAP25 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108990).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)](https://www.abcam.cn/ps/products/226/ab226046/Images/ab226046-322762-anti-snap25-antibody-ep3274-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)ab108990 showing negative staining in Normal human colon tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108990).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)](https://www.abcam.cn/ps/products/226/ab226046/Images/ab226046-322761-anti-snap25-antibody-ep3274-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)ab108990 showing negative staining in Normal human spleen tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108990).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)](https://www.abcam.cn/ps/products/226/ab226046/Images/ab226046-322760-anti-snap25-antibody-ep3274-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)ab108990 showing negative staining in human Skeletal muscle tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab108990).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)](https://www.abcam.cn/ps/products/226/ab226046/Images/ab226046-11-benefits-of-recombinant-antibodies.png)
Anti-SNAP25 antibody [EP3274] - BSA and Azide free (ab226046)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab226046 尚未被引用在任何文献中。
