Anti-SNAP25抗体(ab5666)

Thank you for your recent telephone enquiry.

I can confirm that ab5666 SNAP25 antibodyis provided at 1 mg/ml. I have updated the online datasheet.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

I'm very sorry to hear you are experiencing problems with ab5666 in both WB and IHC, this antibody has not been tested on sections (only in cell culture) and without a detailed IHC protocol it is hard to determine what the problem may be in that application. We have tested the antibody in WB on rat and mouse brain lysate and therefore with the following recommendations I hope this will improve you signal: - use a stronger lysis buffer to extract the protein (e.g RIPA buffer which contains a strong detergent) -incubate the membrane in blocking buffer (5%BSA) in TBST (Tris buffer with Tween 0.1%) for 1 hr then -incubate the membrane in antibody at 1:1000 overnight (diluted in TBST Tween 0.1%) at 4C Please rinse in TBST too and check that your secondary antibody works well with other primary antibodies. These steps will promote the antibody binding and enhance the signal. If you would like me to look at your IHC protocol I enclose a link to the IHC protocol questionnaire, if you put it to my attention I will look at this as soon as I receive it: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=5666&mode=questionaire Please let me know if you need further assistance,