Anti-SNAP25抗体(ab41726)
Key features and details
- Rabbit polyclonal to SNAP25
- Suitable for: IP, ICC/IF, WB
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SNAP25抗体
参阅全部 SNAP25 一抗 -
描述
兔多克隆抗体to SNAP25 -
宿主
Rabbit -
经测试应用
适用于: IP, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human, Zebrafish
预测可用于: Chicken, Cow, Drosophila melanogaster
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- This antibody gave a positive signal in the following lysates: Spinal Cord (Mouse) Tissue Lysate, Spinal Cord (Rat) Tissue lysate, Hippocampus (Mouse) Tissue Lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41726于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use a concentration of 5 µg/ml.
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
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| 说明 |
|---|
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IP
Use a concentration of 5 µg/ml. |
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ICC/IF
Use a concentration of 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa). |
靶标
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功能
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. -
组织特异性
Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. -
序列相似性
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
翻译后修饰
Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. -
细胞定位
Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. - Information by UniProt
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数据库链接
- Entrez Gene: 396444 Chicken
- Entrez Gene: 540853 Cow
- Entrez Gene: 3355084 Drosophila melanogaster
- Entrez Gene: 6616 Human
- Entrez Gene: 20614 Mouse
- Entrez Gene: 25012 Rat
- Entrez Gene: 30712 Zebrafish
- Omim: 600322 Human
see all -
别名
- bA416N4.2 antibody
- Bdr antibody
- CMS18 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody (ab41726)
ab41726 staining SNAP25 in human SH-SY5Y cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab41726 at 5µg/ml and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at 2µg/ml (shown in red). The secondary antibody (shown in green) was ab150081, Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Western blot - Anti-SNAP25 antibody (ab41726)All lanes : Anti-SNAP25 antibody (ab41726) at 1 µg/ml
Lane 1 : Spinal Cord (Mouse) Tissue Lysate
Lane 2 : Spinal Cord (Rat) Tissue lysate
Lane 3 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-SNAP25 antibody (ab41726)ICC/IF image of ab41726 stained human SH-SY5Y cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab41726, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). -
Western blot - Anti-SNAP25 antibody (ab41726)All lanes : Anti-SNAP25 antibody (ab41726) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : SH-SY5Y (Human neuroblastoma cell line) whole cell lysate at 20 µg
Lane 4 : Mouse brain homogenate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted? -
Immunoprecipitation - Anti-SNAP25 antibody (ab41726)SNAP25 was immunoprecipitated using 0.5mg Rat Spinal Cord tissue lysate, 5µg of Rabbit polyclonal to SNAP25 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Rat Spinal Cord tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab41726.
Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
Band: 26kDa; SNAP25; non specific bands - 55 and 98kDa: We are unsure as to the identity of this extra band.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab41726 尚未被引用在任何文献中。