重组Anti-SNAP23抗体[EPR8539] (ab133703)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8539] to SNAP23
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-SNAP23抗体[EPR8539]
参阅全部 SNAP23 一抗 -
描述
兔单克隆抗体[EPR8539] to SNAP23 -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat -
免疫原
Synthetic peptide within Human SNAP23 aa 150-250 (C terminal). The exact sequence is proprietary.
Database link: O00161 -
阳性对照
- Lysates of: fetal liver, HeLa, HepG2, BxPC-3
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 2.50 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR8539 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133703于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
靶标
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功能
Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion. -
组织特异性
Ubiquitous. Highest levels where found in placenta. -
序列相似性
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
细胞定位
Cell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8773 Human
- Entrez Gene: 20619 Mouse
- Entrez Gene: 64630 Rat
- Omim: 602534 Human
- SwissProt: O00161 Human
- SwissProt: O09044 Mouse
- SwissProt: O70377 Rat
- Unigene: 511149 Human
see all -
别名
- HsT17016 antibody
- LS-B8340 antibody
- SNAP 23 antibody
see all
图片
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SNAP23 knockout HAP1 cell lysate (20 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab133703 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133703 was shown to specifically react with SNAP23 when SNAP23 knockout samples were used. Wild-type and SNAP23 knockout samples were subjected to SDS-PAGE. ab133703 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-SNAP23 antibody [EPR8539] (ab133703) at 1/1000 dilution
Lane 1 : Fetal liver cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : BxPC-3 cell lysate
Predicted band size: 23 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab133703 被引用在 1 文献中.
- Lim YS et al. ADP-ribosylation Factor-related Protein 1 Interacts with NS5A and Regulates Hepatitis C Virus Propagation. Sci Rep 6:31211 (2016). PubMed: 27550144