为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Neuroscience Neurotransmission Secretory Vesicles SNAPs & SNAREs

Anti-SNAP23抗体(ab4114)

  • Datasheet
Reviews (2)Q&A (3)References (21)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab4114)

    Key features and details

    • Rabbit polyclonal to SNAP23
    • Suitable for: IHC-Fr, IP, WB, ICC/IF, IHC-P
    • Reacts with: Mouse, Rat, Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-SNAP23 antibody [EPR8538] (ab131242)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术,可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-SNAP23抗体
      参阅全部 SNAP23 一抗
    • 描述

      兔多克隆抗体to SNAP23
    • 宿主

      Rabbit
    • 特异性

      This antibody reacts specifically with human SNAP23.
    • 经测试应用

      适用于: IHC-Fr, IP, WB, ICC/IF, IHC-Pmore details
    • 种属反应性

      与反应: Mouse, Rat, Human
    • 免疫原

      Fusion protein corresponding to Human SNAP23. Immunogen is a SNAP23-GST fusion protein
      Database link: O00161

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Constituent: Whole serum
    • Concentration information loading...
    • 纯度

      Whole antiserum
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Neuroscience
      • Neurotransmission
      • Secretory Vesicles
      • SNAPs & SNAREs
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human SNAP23 protein (ab79180)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab4114于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-Fr
    Use at an assay dependent concentration. PubMed: 17272274
    IP
    Use at an assay dependent concentration. PubMed: 22393054
    WB (1)
    Use at an assay dependent concentration.
    ICC/IF (1)
    Use at an assay dependent concentration.
    IHC-P
    1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    说明
    IHC-Fr
    Use at an assay dependent concentration. PubMed: 17272274
    IP
    Use at an assay dependent concentration. PubMed: 22393054
    WB
    Use at an assay dependent concentration.
    ICC/IF
    Use at an assay dependent concentration.
    IHC-P
    1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    靶标

    • 功能

      Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
    • 组织特异性

      Ubiquitous. Highest levels where found in placenta.
    • 序列相似性

      Belongs to the SNAP-25 family.
      Contains 2 t-SNARE coiled-coil homology domains.
    • 细胞定位

      Cell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.
    • Target information above from: UniProt accession O00161 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 8773 Human
      • Entrez Gene: 20619 Mouse
      • Entrez Gene: 64630 Rat
      • Omim: 602534 Human
      • SwissProt: O00161 Human
      • SwissProt: O09044 Mouse
      • SwissProt: O70377 Rat
      • Unigene: 511149 Human
      • Unigene: 245715 Mouse
      • Unigene: 14789 Rat
      see all
    • 别名

      • HsT17016 antibody
      • LS-B8340 antibody
      • SNAP 23 antibody
      • SNAP-23 antibody
      • SNAP23 antibody
      • SNAP23A antibody
      • SNAP23B antibody
      • SNP23_HUMAN antibody
      • Synaptosomal associated protein 23 antibody
      • Synaptosomal associated protein 23kDa antibody
      • Synaptosomal associated protein antibody
      • Synaptosomal-associated protein 23 antibody
      • Vesicle membrane fusion protein SNAP 23 antibody
      • Vesicle membrane fusion protein SNAP23 antibody
      • Vesicle-membrane fusion protein SNAP-23 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab4114)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab4114)

      IHC image of ab4114 staining in human placenta tissue formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4114, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet download

      Download

    文献 (21)

    发表研究结果有使用 ab4114?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4114 被引用在 21 文献中.

    • Hu CY  et al. Long non-coding RNA NORAD promotes the prostate cancer cell extracellular vesicle release via microRNA-541-3p-regulated PKM2 to induce bone metastasis of prostate cancer. J Exp Clin Cancer Res 40:98 (2021). PubMed: 33722248
    • Qian L  et al. Reduced O-GlcNAcylation of SNAP-23 promotes cisplatin resistance by inducing exosome secretion in ovarian cancer. Cell Death Discov 7:112 (2021). PubMed: 34001861
    • Sikorra S  et al. Engineering an Effective Human SNAP-23 Cleaving Botulinum Neurotoxin A Variant. Toxins (Basel) 12:N/A (2020). PubMed: 33352834
    • Xiong X  et al. Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins. Biol Open 8:N/A (2019). PubMed: 31142470
    • Stévenin V  et al. Dynamic Growth and Shrinkage of the Salmonella-Containing Vacuole Determines the Intracellular Pathogen Niche. Cell Rep 29:3958-3973.e7 (2019). PubMed: 31851926
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-SNAP23 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Lung)
    Loading amount
    20 µg
    Specification
    Lung
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mrs. Katelyn Miller

    Verified customer

    提交于 Jun 25 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SNAP23 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (neurospheres)
    Specification
    neurospheres
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton-x
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Sep 05 2007

    Question

    I was concerned about cross-reactivity with mouse or other rodent SNAP23 proteins. Since we are working with the mouse this information would be very valuable before getting this reagent. Is an other way would consist to test ourselves if you were open to send us a small aliquot?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 07 2002

    Answer

    I'm sorry to inform you have we have no data for the cross reactivity of this anitbody with mouse or other rodent synaptosome associated protein 23. I am not familiar with the degree of sequence homology between human and murine SNAP23. However, as the antibody is a polyclonal it will recognise multiple epitopes across SNAP23 and is therefore more likely to cross react with the protein from different species. If you are still interested in this antibody, please contact me directly. Danielle N. Miller PhD Head of Customer Services danielle.miller@abcam.com

    Read More

    Danielle Miller

    Abcam Scientific Support

    回复于 Mar 28 2002

    Question

    Is there a positive control available for use in WB with this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 26 2001

    Answer

    No, sorry. The only control is with the endogenous protein from the cell line described in the data sheet.

    Read More

    Neil Hayward

    Abcam Scientific Support

    回复于 Jul 27 2001

    Question

    Dear Sir anti snap-23 reacts human SNAP-23, how about rat or mouse? I want to apply against rat homogenate.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 02 2001

    Answer

    This antibody has not yet been tested for cross-reactivity against rat or mouse SNAP-23.

    Read More

    Abcam Scientific Support

    回复于 Apr 02 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2023 艾博抗(上海)贸易有限公司版权所有