AB78105

Anti-SNAIL + SLUG抗体[20C8]

Anti-SNAIL + SLUG antibody [20C8]

(2 Reviews)

(5 Publications)

Mouse Monoclonal SLUG antibody 20C8. Suitable for WB, sELISA and reacts with Human samples. Cited in 5 publications.

查看别名

SLUG, SLUGH, SNAI2, Zinc finger protein SNAI2, Neural crest transcription factor Slug, Protein snail homolog 2

2 Images

Sandwich ELISA - Anti-SNAIL + SLUG antibody [20C8] (AB78105)

sELISA

Unknown

Sandwich ELISA - Anti-SNAIL + SLUG antibody [20C8] (AB78105)

Standard Curve for SNAIL; dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [20C8] to SNAIL + SLUG (ab78105) at 1μg/ml and Detector Antibody Rabbit polyclonal to SNAIL + SLUG - ChIP Grade (ab85931) at 0.5μg/ml.

Unknown

Western blot - Anti-SNAIL + SLUG antibody [20C8] (AB78105)

ab78105 recognizes the tagged recombinant SLUG and SNAIL proteins which have an expected molecular weight of 68 kDa

All lanes:

Western blot - Anti-SNAIL + SLUG antibody [20C8] (ab78105) at 1/250 dilution

Lane 1:

SNAIL (SNAI1) Human Recombinant Protein at 0.1 µg

Lane 2:

SLUG (SNAI2) Human Recombinant Protein at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 68 kDa

true

Exposure time: 3min

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

20C8

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

WB, sELISA

applications

免疫原

Recombinant Full Length Protein corresponding to Human SNAI2.

O43623

特异性

This antibody detects endogonous proteins in the following cell lines: MDAMB-453; MDAMB-435; MDAMB-231; A375.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250", "WB-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "1 µg/mL", "sELISA-species-notes": "<p>For sandwich ELISA, use this antibody as Capture at 1 µg/ml with Rabbit polyclonal to SNAIL - ChIP Grade (<a href='/products/unavailable/snail-slug-antibody-chip-grade-ab85931'>ab85931</a>) as Detection.</p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A/G

存储溶液

Preservative: 0.02% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SNAIL and SLUG also known as Snai1 and Snai2 are zinc finger transcription factors important in the regulation of epithelial-mesenchymal transition (EMT). These proteins are expressed mainly in various developing tissues and tumors. The molecular weight of SNAIL is approximately 29 kDa while SLUG typically has a mass around 30 kDa. These factors function within the nucleus binding to E-box sequences in DNA to repress the transcription of epithelial markers.

Biological function summary

SNAIL and SLUG operate as vital regulators of cell motility and invasion by modulating gene expression. They do not work alone; they participate as components of a complex with other EMT-inducing transcription factors including TWIST and ZEB. These factors suppress E-cadherin and other adhesion molecules which facilitates the disassembly of tight junctions between cells promoting a mesenchymal phenotype that is more migratory and invasive.

Pathways

These transcription factors play key roles in both the TGF-beta and Wnt signaling pathways. In the TGF-beta pathway SNAIL and SLUG regulate gene expression to support EMT affecting processes in development and cancer progression. Within the Wnt pathway these factors interact with beta-catenin impacting cell cycle regulation and supporting cells' transition between epithelial and mesenchymal states a critical step in tumor metastasis.

SNAIL and SLUG significantly relate to cancer and fibrosis. In cancer their interaction with proteins such as ZEB and TWIST is important for the metastasis of carcinoma cells. In fibrosis these transcription factors link to TGF-beta-induced fibroblast activation contributing to tissue scarring. The dysregulation of SNAIL and SLUG often serves as an indicator of poor prognosis due to their roles in promoting invasive and metastatic properties in cancer.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis.

See full target information SNAI2

其他靶点

SNAI1

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Cellular signalling 75:109768 PubMed32896607

2020

Activation of autophagy inhibits epithelial to mesenchymal transition process of human lens epithelial cells induced by high glucose conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Li,Wei Ye,Wenqin Xu,Tianfang Chang,Luning Zhang,Jiyuan Ma,Rui Pei,Mengmei He,Jian Zhou

International journal of molecular medicine 45:265-273 PubMed31746377

2019

SNAI1 interacts with HDAC1 to control TGF‑β2‑induced epithelial‑mesenchymal transition in human lens epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Gao,Jingming Li,Yazhou Qin,Yingna Wang,Qianyang Kang,Cheng Pei

Experimental & molecular medicine 50:e420 PubMed29303507

2018

T-cell immune regulator 1 enhances metastasis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hee Doo Yang,Jung Woo Eun,Kyung-Bun Lee,Qingyu Shen,Hyung Seok Kim,Sang Yean Kim,Dong-Wan Seo,Won Sang Park,Jung Young Lee,Suk Woo Nam

Stem cell research 17:62-8 PubMed27240252

2016

The mesenchymal transcription factor SNAI-1 instructs human liver specification.

Applications

ChIP

Species

Human

Orit Goldman,Victor Julian Valdes,Elena Ezhkova,Valerie Gouon-Evans

Cell death & disease 6:e1801 PubMed26136074

2015

Identification of thiostrepton as a novel therapeutic agent that targets human colon cancer stem cells.

Applications

Species

Unspecified reactive species

S-Y Ju,C-Y F Huang,W-C Huang,Y Su

View all publications

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Anti-SNAIL + SLUG抗体[20C8]

Anti-SNAIL + SLUG antibody [20C8]

Sandwich ELISA - Anti-SNAIL + SLUG antibody [20C8] (AB78105)

Western blot - Anti-SNAIL + SLUG antibody [20C8] (AB78105)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性