Anti-SNAIL抗体[Sn9H2] (ab31787)
![Western blot - Anti-SNAIL antibody [Sn9H2] (ab31787)](https://www.abcam.cn/ps/products/31/ab31787/Images/ab31787-42947-anti-snail-antibody-sn9h2-western-blot.jpg "Western blot - Anti-SNAIL antibody [Sn9H2] (ab31787)")
Key features and details
- Rat monoclonal [Sn9H2] to SNAIL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-SNAIL抗体[Sn9H2]
参阅全部 SNAIL 一抗 -
描述
大鼠单克隆抗体[Sn9H2] to SNAIL -
宿主
Rat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Fusion protein corresponding to Human SNAIL.
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阳性对照
- This antibody gave a positive signal when tested against the SNAIL recombinant protein.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
Sn9H2 -
骨髓瘤
P3x63-Ag8.653 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31787于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 10 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 10 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). |
靶标
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功能
Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself. -
组织特异性
Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines. -
序列相似性
Belongs to the snail C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers. -
翻译后修饰
Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation. -
细胞定位
Nucleus. Cytoplasm. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs. - Information by UniProt
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数据库链接
- Entrez Gene: 6615 Human
- Omim: 604238 Human
- SwissProt: O95863 Human
- Unigene: 48029 Human
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别名
- dJ710H13.1 antibody
- Protein sna antibody
- Protein snail homolog 1 antibody
see all
图片
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Western blot - Anti-SNAIL antibody [Sn9H2] (ab31787)All lanes : Anti-SNAIL antibody [Sn9H2] (ab31787) at 10 µg/ml
Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
Lane 2 : SLUG (SNAI2) Human Recombinant Protein
Lysates/proteins at 0.1 µg per lane.
Secondary
All lanes : Peroxidase Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?
Exposure time: 16 minutes
Ab31787 recognizes the tagged recombinant SNAIL protein which has an expected molecular weight of 68 kDa.
数据表及文件
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Datasheet download
文献 (8)
ab31787 被引用在 8 文献中.
- Zhang X et al. Over-Expression of ARID3B Suppresses Tumor Progression and Predicts Better Prognosis in Patients With Gastric Cancer. Cancer Control 30:10732748231169403 (2023). PubMed: 37071790
- Yi JM et al. Human Neuralized is a novel tumour suppressor targeting Wnt/β-catenin signalling in colon cancer. EMBO Rep 24:e56335 (2023). PubMed: 37341560
- Zhang Y et al. LINC00891 regulated by miR-128-3p/GATA2 axis impedes lung cancer cell proliferation, invasion and EMT by inhibiting RhoA pathway. Acta Biochim Biophys Sin (Shanghai) 54:378-387 (2022). PubMed: 35538035
- Tang Z et al. Enhanced monoacylglycerol lipolysis by ABHD6 promotes NSCLC pathogenesis. EBioMedicine 53:102696 (2020). PubMed: 32143183
- Wang S et al. MiR-193a-5p suppresses cell proliferation and induces cell apoptosis by regulating HOXA7 in human ovarian cancer. Neoplasma 67:825-833 (2020). PubMed: 32305054
- Weng W et al. Metastasis-associated protein 1 promotes tumor invasion by downregulation of E-cadherin. Int J Oncol 44:812-8 (2014). Human . PubMed: 24424621
- Fedorova L et al. Down-regulation of the transcription factor snail in the placentas of patients with preeclampsia and in a rat model of preeclampsia. Reprod Biol Endocrinol 10:15 (2012). PubMed: 22360878
- Rosivatz E et al. Expression and nuclear localization of Snail, an E-cadherin repressor, in adenocarcinomas of the upper gastrointestinal tract. Virchows Arch 448:277-87 (2006). PubMed: 16328348
