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使用敲除细胞株进行验证重组RabMAb

重组Anti-SMYD3抗体[EPR11107(2)] (ab187149)

Submit a review Submit a question References (15)
Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
  • Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
  • Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
  • Flow Cytometry (Intracellular) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
  • Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
  • Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
  • Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11107(2)] to SMYD3
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase APC Carrier Free HRP PE

概述

  • 产品名称

    Anti-SMYD3抗体[EPR11107(2)]
    参阅全部 SMYD3 一抗

  • 描述

    兔单克隆抗体[EPR11107(2)] to SMYD3

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Wild-type HAP1, MCF7, HeLa, HEK-293, T47-D and NIH/3T3 cell lysates; Mouse brain and Rat spleen tissue lysates. IHC-P: Human colonic carcinoma and mouse skeletal muscle tissues. ICC/IF: MCF7 and HeLa cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab187149于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/20000. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250.
IP
1/40 - 1/60.
说明
Flow Cyt (Intra)
1/30.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/20000. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250.
IP
1/40 - 1/60.

靶标

  • 功能

    Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.

  • 组织特异性

    Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.

  • 序列相似性

    Belongs to the histone-lysine methyltransferase family.
    Contains 1 MYND-type zinc finger.
    Contains 1 SET domain.

  • 细胞定位

    Cytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.
  • Information by UniProt

  • 数据库链接

    • 别名

      • bA74P14.1 (novel protein) antibody
      • bA74P14.1 antibody
      • FLJ21080 antibody
      • histone lysine N methyltransferase SMYD3 antibody
      • KMT3E antibody
      • MGC104324 antibody
      • SET and MYND domain containing 3 antibody
      • SET and MYND domain containing protein 3 antibody
      • SET and MYND domain-containing protein 3 antibody
      • SMYD 3 antibody
      • Smyd3 antibody
      • SMYD3 protein antibody
      • SMYD3_HUMAN antibody
      • Zinc finger MYND domain containing 1 antibody
      • Zinc finger MYND domain containing protein 1 antibody
      • Zinc finger MYND domain-containing protein 1 antibody
      • Zinc finger protein subfamily 3A MYND domain containing 1 antibody
      • Zinc finger protein, subfamily 3A (MYND domain containing), 1 antibody
      • ZMYND 1 antibody
      • ZMYND1 antibody
      • ZNFN3A1 antibody
      see all

    图片

    • Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
      Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
      All lanes : Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : SMYD3 knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : MCF7 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 49 kDa
      Observed band size: 49 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab187149 observed at 49 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

      ab187149 was shown to react with SMYD3 in HAP1 wild-type cells in Western blot. Loss of signal was observed when SMYD3 knockout sample was used. HAP1 wild-type and SMYD3 knockout whole cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab187149 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
      Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
      All lanes : Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/20000 dilution

      Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
      Lane 4 : T47-D (human ductal breast epithelial tumor cell line) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 49 kDa



      Blocking and dilution buffer: 5% NFDM/TBST

    • Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
      Immunocytochemistry/ Immunofluorescence - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells, labeling SMYD3 with ab187149 at 1/250 dilution (left image; green); secondary antibody was Goat anti rabbit IgG Alexa Fluor®488, and counterstained with Dapi (right image; blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

      Immunohistochemical analysis of paraffin-embedded human colonic carcinoma tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
      Flow Cytometry (Intracellular) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

      Intracellular flow cytometric analysis of 2%paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, labeling SMYD3 with ab187149 at 1/30 dilution. Secondary antibody wasGoat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control was a Rabbit monoclonal IgG. 

       

       

    • Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
      Immunoprecipitation - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

      Immunoprecipitation analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate labeling SMYD3 using ab187149 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of HeLa lysate.

    • Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
      Western blot - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
      All lanes : Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/5000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat spleen tissue lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 49 kDa



      Blocking and dilution buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

      Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-SMYD3 antibody [EPR11107(2)] (ab187149)
      Anti-SMYD3 antibody [EPR11107(2)] (ab187149)

    文献 (15)

    发表研究结果有使用 ab187149？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab187149 被引用在 15 文献中.

    • Yang Z  et al. Histone lysine methyltransferase SMYD3 promotes oral squamous cell carcinoma tumorigenesis via H3K4me3-mediated HMGA2 transcription. Clin Epigenetics 15:92 (2023). PubMed: 37237385
    • Zeng Y  et al. SMYD3 drives the proliferation in gastric cancer cells via reducing EMP1 expression in an H4K20me3-dependent manner. Cell Death Dis 14:386 (2023). PubMed: 37386026
    • Lee B  et al. The long non-coding RNA SPRIGHTLY and its binding partner PTBP1 regulate exon 5 skipping of SMYD3 transcripts in group 4 medulloblastomas. Neurooncol Adv 4:vdac120 (2022). PubMed: 36267874
    • Lv HW  et al. SMYD3 confers cisplatin chemoresistance of NSCLC cells in an ANKHD1-dependent manner. Transl Oncol 14:101075 (2021). PubMed: 33773404
    • Wang Q  et al. Chitooligosaccharides Modulate Glucose-Lipid Metabolism by Suppressing SMYD3 Pathways and Regulating Gut Microflora. Mar Drugs 18:N/A (2020). PubMed: 31968646
    View all Publications for this product

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