重组Anti-SMYD3抗体[EPR11106(2)] - N-terminal (ab183498)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11106(2)] to SMYD3 - N-terminal
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SMYD3抗体[EPR11106(2)] - N-terminal
参阅全部 SMYD3 一抗 -
描述
兔单克隆抗体[EPR11106(2)] to SMYD3 - N-terminal -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, HAP1, 293, MCF-7 and T47-D cell lysates. IHC-P: Human testis and skeletal muscle tissue. ICC: HeLa cells. Flow Cyt (intra): 293 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11106(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183498于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).
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ICC/IF |
1/500.
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IP |
1/40 - 1/60.
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说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa). |
ICC/IF
1/500. |
IP
1/40 - 1/60. |
靶标
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功能
Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences. -
组织特异性
Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas. -
序列相似性
Belongs to the histone-lysine methyltransferase family.
Contains 1 MYND-type zinc finger.
Contains 1 SET domain. -
细胞定位
Cytoplasm. Nucleus. Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M. - Information by UniProt
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数据库链接
- Entrez Gene: 64754 Human
- Omim: 608783 Human
- SwissProt: Q9H7B4 Human
- Unigene: 567571 Human
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别名
- bA74P14.1 (novel protein) antibody
- bA74P14.1 antibody
- FLJ21080 antibody
see all
图片
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All lanes : Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : SMYD3 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 49 kDaLanes 1 - 4: Merged signal (red and green). Green - ab183498 observed at 49 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab183498 was shown to recognize SMYD3 in wild-type HAP1 cells as signal was lost at the expected MW in SMYD3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SMYD3 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab183498 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/10000 dilution
Lane 1 : HeLa lysate
Lane 2 : 293 lysate
Lane 3 : T47-D lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 49 kDa -
Immunohistochemical analysis of paraffin-embedded Human skeletal muscle labeling SMYD3 at 1/100 dilution and HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Flow cytometric analysis of HeLa cells fixed in 20 °C acetone labeling SMYD3 at 1/500 dilution and Goat anti rabbit IgG(Alexa Fluor® 555) at 1/200 (red). Counterstained with DAPI (blue)
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Immunoprecipitation of HeLa cells labeling SMYD3 with ab183498 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.
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Intracellular flow cytometric analysis of 293 cells fixed in 2% paraformaldehyde labeling SMYD3 with ab183498 at 1/110 dilution andGoat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit Monoclonal IgG was used as an isotype control.
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Anti-SMYD3 antibody [EPR11106(2)] - N-terminal (ab183498) at 1/2000 dilution + MCF-7 lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 49 kDa -
Immunohistochemical analysis of paraffin-embedded Human testis labeling SMYD3 with ab183498 at 1/100 dilution and HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (3)
ab183498 被引用在 3 文献中.
- Lee B et al. The long non-coding RNA SPRIGHTLY and its binding partner PTBP1 regulate exon 5 skipping of SMYD3 transcripts in group 4 medulloblastomas. Neurooncol Adv 4:vdac120 (2022). PubMed: 36267874
- Alshiraihi IM et al. In Silico/In Vitro Hit-to-Lead Methodology Yields SMYD3 Inhibitor That Eliminates Unrestrained Proliferation of Breast Carcinoma Cells. Int J Mol Sci 21:N/A (2020). PubMed: 33333978
- McNulty DE et al. A High-Throughput Dose-Response Cellular Thermal Shift Assay for Rapid Screening of Drug Target Engagement in Living Cells, Exemplified Using SMYD3 and IDO1. SLAS Discov 23:34-46 (2018). PubMed: 28957646