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AB250870

重组Anti-SMYD1抗体[EPR13573] - BSA and Azide free

Anti-SMYD1 antibody [EPR13573] - BSA and Azide free

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Rabbit Recombinant Monoclonal SMYD1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.

查看别名

Histone-lysine N-methyltransferase SMYD1, SET and MYND domain-containing protein 1, SMYD1

2 Images
Western blot - Anti-SMYD1 antibody [EPR13573] - BSA and Azide free (AB250870)
  • WB

Supplier Data

Western blot - Anti-SMYD1 antibody [EPR13573] - BSA and Azide free (AB250870)

This data was developed using ab185969, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SMYD1 antibody [EPR13573] (<a href='/products/primary-antibodies/smyd1-antibody-epr13573-ab185969'>ab185969</a>) at 1/1000 dilution

Lane 1:

Human fetal heart lysate at 20 µg

Lane 2:

Human skeletal muscle lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 56 kDa

true

Western blot - Anti-SMYD1 antibody [EPR13573] - BSA and Azide free (AB250870)
  • WB

Supplier Data

Western blot - Anti-SMYD1 antibody [EPR13573] - BSA and Azide free (AB250870)

This data was developed using ab185969, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SMYD1 antibody [EPR13573] (<a href='/products/primary-antibodies/smyd1-antibody-epr13573-ab185969'>ab185969</a>) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 10 µg

Lane 2:

Rat heart lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa

true

不同偶联物与剂型 (1)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13573

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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产品详情

ab250870 is the carrier-free version of ab185969.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SET and MYND domain-containing protein 1 (SMYD1) also known as BOP is a protein of approximately 56 kDa that functions as a histone methyltransferase. It modifies histones by transferring methyl groups to specific lysine residues particularly histone H3 at lysine 4 (H3K4). SMYD1 expression is mainly observed in cardiac and skeletal muscle tissues. It plays a role in regulating gene expression by altering chromatin structure therefore influencing transcriptional activity.
Biological function summary

SMYD1 plays a significant role in muscle differentiation and development. Its activity leads to chromatin modifications that facilitate muscle-specific gene expression. SMYD1 interacts in a complex network with other proteins involved in muscle function acting as part of the transcriptional machinery that marks certain genes for activation. This methyltransferase activity contributes to the proper formation of muscle fibers.

Pathways

SMYD1 is important in pathways that control heart development and muscle function. It is active in the MAPK signaling pathway which is essential for cell growth and differentiation and interacts with proteins such as MEF2C a transcription factor important for muscle development. This connection influences the regulation and expression of various genes that are critical for normal cardiac and skeletal muscle function.

Mutations or dysregulation of SMYD1 can lead to congenital heart defects and myopathies. Its interactions with proteins like MYH7 a myosin heavy chain associated with proper cardiac muscle function are important in these conditions. Anomalies in SMYD1 activity can disrupt normal cardiac and skeletal muscle development leading to diseases characterized by structural or functional abnormalities of the heart and muscles.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Methylates histone H3 at 'Lys-4' (H3K4me), seems able to perform both mono-, di-, and trimethylation. Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.
See full target information SMYD1
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搭配使用产品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8580

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

  • 5
  • 90
Primary Antibodies

AB184267

Anti-HuD + HuC antibody [EPR19098]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD + HuC antibody [EPR19098] (AB184267)

  • 5
  • 4
Primary Antibodies

AB16287

Anti-SSEA4 antibody [MC813-70]

Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813-70] (AB16287)

  • 4
  • 11

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