Anti-Smt3抗体(ab14405)
Key features and details
- Rabbit polyclonal to Smt3
- Suitable for: WB, ELISA
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
概述
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产品名称
Anti-Smt3抗体 -
描述
兔多克隆抗体to Smt3 -
宿主
Rabbit -
经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Saccharomyces cerevisiae -
免疫原
Recombinant protein (S. cerevisiae).
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阳性对照
- Most yeast cell lysates can be used as a positive control without induction or stimulation.
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常规说明
Centrifuge product if not completely clear after standing at room temperature.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
纯化说明
Anti-Sumo Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14405于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
1/1000. Detects a band of approximately 12 kDa.
Note: Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. |
| ELISA | (1) |
1/1000 - 1/5000.
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| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 12 kDa. Note: Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. |
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ELISA
1/1000 - 1/5000. |
靶标
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功能
Not known; suppressor of MIF2 mutations. -
序列相似性
Belongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain. - Information by UniProt
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数据库链接
- Entrez Gene: 852122 Saccharomyces cerevisiae
- SwissProt: Q12306 Saccharomyces cerevisiae
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别名
- DmSUMO 1 antibody
- Small Ubiquitin-like modifier antibody
- SMT3 antibody
see all
图片
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Western blot - Anti-Smt3 antibody (ab14405)All lanes : Anti-Smt3 antibody (ab14405) at 1/1000 dilution
Lanes 1 & 3 & 5 & 7 & 9 & 11 & 13 : Lysate prepared from E.coli expressed Yeast SUMO protein and SUMO-GFP fusion protein after incubation with lysed insect cells
Lanes 2 & 4 & 6 & 8 & 10 & 12 & 14 : Lysate prepared from SUMO-GFP fusion protein after incubation with lysed insect cells in the presence of 2% SDS
Lysates/proteins at 2 µg per lane.Western blot of SUMO-GFP fusion proteins cleaved by insect cell protein extracts. Anti-SUMO antibody, generated by immunization with recombinant yeast SUMO, was tested by western blot against several constructs of SUMO-GFP fusion proteins after cleavage by proteases in insect cell protein extracts. These constructs contained various linkers between the SUMO and GFP portion of the fusion proteins.
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ELISA - Anti-Smt3 antibody (ab14405)This image is courtesy of an anonymous Abreviewab14405 detecting Smt3 in Escherichia coli recombinant protein (BL21(DE3) by Direct ELISA. Wells were coated with 10µg each of diluted control protein (without SUMO fusion - Negative Controls) and target protein (with SUMO fusion protein) in bicarbonate buffer. Wells were blocked with 5% milk for 1 hour at room temperature. The samples were incubated with primary antibody (1/5000 in TBST +5% milk) for 45 minutes. An HRP-conjugated goat polyclonal to rabbit IgG (1/40000) was used as the secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab14405 被引用在 10 文献中.
- Makri Pistikou AM et al. Engineering a scalable and orthogonal platform for synthetic communication in mammalian cells. Nat Commun 14:7001 (2023). PubMed: 37919273
- Kobayashi T et al. Purification and Characterization of Double-Stranded Nucleic Acid-Dependent ATPase Activities of Tagged Dicer-Related Helicase 1 and its Short Isoform in Caenorhabditis elegans. Genes (Basel) 11:N/A (2020). PubMed: 32630243
- Gutierrez-Escribano P et al. Purified Smc5/6 Complex Exhibits DNA Substrate Recognition and Compaction. Mol Cell 80:1039-1054.e6 (2020). PubMed: 33301732
- Bauer SL et al. Helicase/SUMO-targeted ubiquitin ligase Uls1 interacts with the Holliday junction resolvase Yen1. PLoS One 14:e0214102 (2019). PubMed: 30897139
- Ranjha L et al. Sumoylation regulates the stability and nuclease activity of Saccharomyces cerevisiae Dna2. Commun Biol 2:174 (2019). PubMed: 31098407
- Ainscough JS et al. Cathepsin S is the major activator of the psoriasis-associated proinflammatory cytokine IL-36?. Proc Natl Acad Sci U S A 114:E2748-E2757 (2017). PubMed: 28289191
- Neiswinger J et al. Posttranslational Modification Assays on Functional Protein Microarrays. Cold Spring Harb Protoc 2016:pdb.prot087999 (2016). PubMed: 27698238
- Bermúdez-López M et al. Sgs1's roles in DNA end resection, HJ dissolution, and crossover suppression require a two-step SUMO regulation dependent on Smc5/6. Genes Dev 30:1339-56 (2016). PubMed: 27298337
- Lescasse R et al. End-joining inhibition at telomeres requires the translocase and polySUMO-dependent ubiquitin ligase Uls1. EMBO J 32:805-15 (2013). PubMed: 23417015
- Khan MA et al. Knockout of caspase-like gene, YCA1, abrogates apoptosis and elevates oxidized proteins in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 102:17326-31 (2005). WB ; Saccharomyces cerevisiae . PubMed: 16301538