Name: Anti-Smoothelin antibody [R4A]
SKU: ab8969
Rating: 5 (5 reviews)

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Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Key features and details

Mouse monoclonal [R4A] to Smoothelin
Suitable for: ICC/IF, IHC-Fr, IHC-P, WB
Reacts with: Chicken, Cat, Dog, Human, Pig, Monkey
Isotype: IgG1

产品名称

Anti-Smoothelin抗体[R4A]
参阅全部 Smoothelin 一抗
描述

小鼠单克隆抗体[R4A] to Smoothelin
宿主

Mouse
特异性

Reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells.
经测试应用

适用于: ICC/IF, IHC-Fr, IHC-P, WBmore details
种属反应性

与反应: Chicken, Cat, Dog, Human, Pig, Monkey
不与反应: Mouse, Rat, Hamster
免疫原

Recombinant full length protein corresponding to Chicken Smoothelin. Derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal extract of chicken gizzard.
Database link: A0A8V0ZU10
常规说明

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液

Preservative: 0.09% Sodium azide
Constituent: PBS
Concentration information loading...
纯度

Protein G purified
克隆

单克隆
克隆编号

R4A
骨髓瘤

Sp2/0-Ag14
同种型

IgG1
研究领域

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

The Abpromise guarantee

Abpromise™承诺保证使用ab8969于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
ICC/IF	(1)	Use at an assay dependent concentration.
IHC-Fr		Use at an assay dependent concentration.
IHC-P	(3)	Use at an assay dependent concentration.
WB	(1)	1/100 - 1/1000.

说明
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/1000.

功能

Structural protein of the cytoskeleton.
组织特异性

Smooth muscle; contractile or vascular (for the long form).
序列相似性

Belongs to the smoothelin family.
Contains 1 CH (calponin-homology) domain.
细胞定位

Cytoplasm > cytoskeleton. Exhibits a filamentous organization.
Target information above from: UniProt accession P53814 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 373982 Chicken
- Entrez Gene: 6525 Human
- Omim: 602127 Human
- SwissProt: P53814 Human
- Unigene: 149098 Human
别名
- FLJ35168 antibody
- FLJ35365 antibody
- FLJ35620 antibody
- FLJ38597 antibody
- Smoothelin antibody
- SMSMO antibody
- Smtn antibody
- SMTN_HUMAN antibody
see all

Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).
Western blot - Anti-Smoothelin antibody [R4A] (ab8969)

All lanes : Anti-Smoothelin antibody [R4A] (ab8969)

Lane 1 : Human uterine artery
Lane 2 : Human uterine corpus.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Immunohistochemical analysis on paraffin section of human smooth muscle labeling Smoothelin with ab8969.
Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)

Indirect immunofluorescence staining of a cultured human fibroblast, showing colocalization of smoothelin (red fluorescence; as detected by ab8969) and actin (green fluorescence).
Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).
Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Smooth muscle cells in swine colon as detected by ab8969 (dilution 1:100).
Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)

Human blood vessel in cardiac muscle stained with the monoclonal smoothelin antibody ab8969.
Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Immunohistochemical analysis on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.
Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (ab8969)This image is courtesy of an anonymous Abreview

ab8969 staining cells from a human vascular smooth muscle cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 10 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 21°C. An Rhodamine conjugated donkey anti-mouse antibody, diluted 1/200, was used as the secondary.
See Abreview
Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (ab8969)

Immunohistochemistry on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab8969？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab8969 被引用在 27 文献中.

Ding H et al. Differentially expressed mRNAs and their upstream miR-491-5p in patients with coronary atherosclerosis as well as the function of miR-491-5p in vascular smooth muscle cells. Korean J Physiol Pharmacol 26:183-193 (2022). PubMed: 35477546
Xia Y et al. MIRLET7BHG promotes hepatocellular carcinoma progression by activating hepatic stellate cells through exosomal SMO to trigger Hedgehog pathway. Cell Death Dis 12:326 (2021). PubMed: 33771969
Tao W et al. MicroRNA-199a-5p aggravates angiotensin II-induced vascular smooth muscle cell senescence by targeting Sirtuin-1 in abdominal aortic aneurysm. J Cell Mol Med 25:6056-69 (2021). PubMed: 34132029
Jin YP et al. Encapsulated three-dimensional bioprinted structure seeded with urothelial cells: a new construction technique for tissue-engineered urinary tract patch. Chin Med J (Engl) 133:424-434 (2020). PubMed: 31977553
Ackers I et al. Oxidized Low-Density Lipoprotein Induces WNT5A Signaling Activation in THP-1 Derived Macrophages and a Human Aortic Vascular Smooth Muscle Cell Line. Front Cardiovasc Med 7:567837 (2020). PubMed: 33330641

View all Publications for this product

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Sheep Tissue sections (Carotid Artery)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9.0

Permeabilization

Yes - 0.05% Tween 20

Specification

Carotid Artery

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Le Zhen

Verified customer

提交于 Aug 23 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rabbit Tissue sections (Carotid Artery)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer (pH=6)

Permeabilization

Yes - 0.1% Triton X-100

Specification

Carotid Artery

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Oct 20 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Dog Tissue lysate - other (canine myocardial infarct)

Specification

canine myocardial infarct

Antigen retrieval step

None

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 2%

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Marcin Dobaczewski

Verified customer

提交于 Aug 11 2005

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Key features and details

概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

骨髓瘤

同种型

研究领域

相关产品

Compatible Secondaries

Isotype control

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (27)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smoothelin antibody [R4A]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smoothelin antibody [R4A]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Smoothelin antibody [R4A]