AB8969

Anti-Smoothelin 抗体 [R4A]

Name: Smoothelin抗体[R4A] (ab8969)| Abcam中文官网
Brand: Abcam Limited
SKU: AB8969
Price: 5145 CNY
Availability: InStock
Rating: 5 (5 reviews)

Anti-Smoothelin antibody [R4A]

(5 Reviews)

(32 Publications)

Mouse Monoclonal Smoothelin antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Pig, Chicken, Monkey, Human, Dog, Cat samples. Cited in 32 publications.

查看别名

SMSMO, SMTN, Smoothelin

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [R4A] (AB8969)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [R4A] (AB8969)

Immunohistochemical analysis on paraffin section of human smooth muscle labeling Smoothelin with ab8969.

Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (AB8969)

ICC/IF

AbReview11927****

Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (AB8969)

ab8969 staining cells from a human vascular smooth muscle cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 10 minutes at 21°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 21°C. An Rhodamine conjugated donkey anti-mouse antibody, diluted 1/200, was used as the secondary.

This image is courtesy of an anonymous Abreview

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (AB8969)

Indirect immunofluorescence staining of a cultured human fibroblast, showing colocalization of smoothelin (red fluorescence; as detected by ab8969) and actin (green fluorescence).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (AB8969)

Human blood vessel in cardiac muscle stained with the monoclonal smoothelin antibody ab8969.

Unknown

Western blot - Anti-Smoothelin antibody [R4A] (AB8969)

All lanes:

Western blot - Anti-Smoothelin antibody [R4A] (ab8969)

Lane 1:

Human uterine artery

Lane 2:

Human uterine corpus

Predicted band size: 99 kDa

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Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (AB8969)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (AB8969)

Immunohistochemical analysis on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (AB8969)

Smooth muscle cells in swine colon as detected by ab8969 (dilution 1 : 100).

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (AB8969)

Smooth muscle cells in swine colon as detected by ab8969 (dilution 1 : 100).

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (AB8969)

Smooth muscle cells in swine colon as detected by ab8969 (dilution 1 : 100).

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (AB8969)

Immunohistochemistry on frozen section of chicken gizzard striated muscle labeling Smoothelin with ab8969.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

R4A

亚型

IgG1

不含载体蛋白

反应种属

Chicken, Human, Pig, Cat, Monkey, Dog

应用

ICC/IF, IHC-Fr, WB, IHC-P

applications

特异性

Reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Chicken": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Hamster": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Monkey": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

存储溶液

Preservative: 0.09% Sodium azide Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Smoothelin is a cytoskeletal protein known for its mechanical role in smooth muscle cells. It is approximately 59 kDa in mass. The protein binds to actin filaments contributing to muscle cell contractility and maintaining the integrity of vascular tissues. It is expressed predominantly in contractile smooth muscle cells in tissues such as the intestines bladder and vasculature. Alternative names for Smoothelin include SMTN and smoothelin-A the latter referring to its variant expressed in some muscle tissues.

Biological function summary

Smoothelin is an important component in smooth muscle contraction processes. It is not typically associated with larger protein complexes instead it functions directly with actin filaments. The protein stabilizes and organizes these filaments facilitating a contracted state that is essential for the function of hollow organs and blood vessels. Through its interactions with actin Smoothelin plays a regulatory role in ensuring the contractile apparatus of smooth muscle tissues functions properly.

Pathways

Smoothelin plays an integral role in the smooth muscle contractile pathway. This pathway includes interactions with other proteins such as myosin light-chain kinase which phosphorylates myosin to facilitate muscle contraction. Additionally Smoothelin is associated with the actin cytoskeleton pathway which orchestrates the organization of the cytoskeleton to manage cellular shape and motility. These pathways highlight the coordination among actin-binding proteins in supporting dynamic cellular functions.

Dysregulation or alteration of Smoothelin expression associates with conditions like atherosclerosis and bladder dysfunction. In atherosclerosis impaired smooth muscle contractility often related to aberrant protein expression contributes to vascular stiffness and plaque formation. Furthermore changes in Smoothelin expression in bladder tissues can link to smooth muscle dysfunction disorders. Smoothelin's activity may influence or be influenced by other proteins involved in these diseases such as myosin light-chain phosphatase which counterbalances kinase activity to regulate contraction levels.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Structural protein of the cytoskeleton.

See full target information SMTN

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文献 (32)

Recent publications for all applications. Explore the full list and refine your search

Lipids in health and disease 24:239 PubMed40660209

2025

Lipid infiltration promotes trans-differentiation of vascular smooth muscle cells into macrophage-like cells in early lesions of human coronary atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Junhai Hao,Jiang Liu,Jiahui Zhou,Yuanfeng Liang,Wanwen Chen,Yueheng Wu,Zhanyi Lin

Cardiovascular toxicology 24:889-903 PubMed39138741

2024

Ciprofloxacin Accelerates Angiotensin-II-Induced Vascular Smooth Muscle Cells Senescence Through Modulating AMPK/ROS pathway in Aortic Aneurysm and Dissection.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyue Zeng,Yaowen Liang,Shangjun Huang,Jiarui Zhang,Cong Mai,Binbin He,Linli Shi,Baojuan Liu,Weifeng Li,Xiaoran Huang,Xin Li

Aging cell 23:e14150 PubMed38576084

2024

Progeria-based vascular model identifies networks associated with cardiovascular aging and disease.

Applications

Unspecified application

Species

Unspecified reactive species

Mzwanele Ngubo,Zhaoyi Chen,Darin McDonald,Rana Karimpour,Amit Shrestha,Julien Yockell-Lelièvre,Aurélie Laurent,Ojong Tabi Ojong Besong,Eve C Tsai,F Jeffrey Dilworth,Michael J Hendzel,William L Stanford

Biomolecules 13: PubMed37509110

2023

Apolipoprotein E (ApoE) Rescues the Contractile Smooth Muscle Cell Phenotype in Popliteal Artery Aneurysm Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Pauli,Tessa Reisenauer,Greg Winski,Nadja Sachs,Ekaterina Chernogubova,Hannah Freytag,Christoph Otto,Christian Reeps,Hans-Henning Eckstein,Claus-Jürgen Scholz,Lars Maegdefessel,Albert Busch

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 26:183-193 PubMed35477546

2022

Differentially expressed mRNAs and their upstream miR-491-5p in patients with coronary atherosclerosis as well as the function of miR-491-5p in vascular smooth muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Ding,Quanhua Pan,Long Qian,Chuanxian Hu

Journal of cellular and molecular medicine 25:6056-6069 PubMed34132029

2021

MicroRNA-199a-5p aggravates angiotensin II-induced vascular smooth muscle cell senescence by targeting Sirtuin-1 in abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Wuyuan Tao,Yimei Hong,Haiwei He,Qian Han,Mengmeng Mao,Bei Hu,Hao Zhang,Xiaoran Huang,Wei You,Xiaoting Liang,Yuelin Zhang,Xin Li

Cell death & disease 12:326 PubMed33771969

2021

MIRLET7BHG promotes hepatocellular carcinoma progression by activating hepatic stellate cells through exosomal SMO to trigger Hedgehog pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunhong Xia,Lu Zhen,Hongxia Li,Shuomin Wang,Sun Chen,Chongchong Wang,Xiaoyu Yang

Frontiers in cardiovascular medicine 7:567837 PubMed33330641

2020

Oxidized Low-Density Lipoprotein Induces WNT5A Signaling Activation in THP-1 Derived Macrophages and a Human Aortic Vascular Smooth Muscle Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Ian Ackers,Candice Szymanski,Mitchell J Silver,Ramiro Malgor

Experimental and therapeutic medicine 19:2913-2922 PubMed32256776

2020

Ginsenoside Rh2 activates α-catenin phosphorylation to inhibit lung cancer cell proliferation and invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Guodong Zhang,Lixiang He,Junhao Chen,Botao Xu,Zejun Mao

Chinese medical journal 133:424-434 PubMed31977553

2020

Encapsulated three-dimensional bioprinted structure seeded with urothelial cells: a new construction technique for tissue-engineered urinary tract patch.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Peng Jin,Chong Shi,Yuan-Yi Wu,Ji-Lei Sun,Jiang-Ping Gao,Yong Yang

View all publications

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Anti-Smoothelin 抗体 [R4A]

Anti-Smoothelin antibody [R4A]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smoothelin antibody [R4A] (AB8969)

Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (AB8969)

Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (AB8969)

Immunocytochemistry/ Immunofluorescence - Anti-Smoothelin antibody [R4A] (AB8969)

Western blot - Anti-Smoothelin antibody [R4A] (AB8969)

Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (AB8969)

Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (AB8969)

Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (AB8969)

Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (AB8969)

Immunohistochemistry (Frozen sections) - Anti-Smoothelin antibody [R4A] (AB8969)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

特异性