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重组RabMAb

重组Anti-smooth muscle Myosin heavy chain 11抗体[EPR5336(B)] (ab133567)

Reviews (2) Submit a question References (16)
Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Flow Cytometry (Intracellular) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
  • Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5336(B)] to smooth muscle Myosin heavy chain 11
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

概述

  • 产品名称

    Anti-smooth muscle Myosin heavy chain 11抗体[EPR5336(B)]
    参阅全部 smooth muscle Myosin heavy chain 11 一抗

  • 描述

    兔单克隆抗体[EPR5336(B)] to smooth muscle Myosin heavy chain 11

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC: Human ovary and prostate tissues; WB: Human bladder, artery and testis tissue lysates; Flow Cyt (intra): 293 cells; ICC/IF: Human smooth muscle and A673 cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab133567于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/20.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 227 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100-1/250
ICC/IF (1)
1/50.
说明
Flow Cyt (Intra)
1/20.
WB
1/1000 - 1/10000. Predicted molecular weight: 227 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100-1/250
ICC/IF
1/50.

靶标

  • 功能

    Muscle contraction.

  • 组织特异性

    Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.

  • 疾病相关

    Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.

  • 序列相似性

    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.

  • 结构域

    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

  • 细胞定位

    Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AAT4 antibody
      • DKFZp686D10126 antibody
      • DKFZp686D19237 antibody
      • FAA4 antibody
      • FLJ35232 antibody
      • MGC126726 antibody
      • MGC32963 antibody
      • MYH 11 antibody
      • MYH11 antibody
      • MYH11_HUMAN antibody
      • Myosin 11 antibody
      • Myosin heavy chain 11 antibody
      • Myosin heavy chain 11 smooth muscle antibody
      • Myosin heavy chain antibody
      • Myosin heavy chain smooth muscle isoform antibody
      • Myosin heavy polypeptide 11 smooth muscle antibody
      • Myosin-11 antibody
      • SMHC antibody
      • SMMHC antibody
      • smooth muscle isoform antibody
      • Smooth muscle myosin heavy chain 11 isoform SM2 antibody
      • Smooth muscle myosin heavy chain isoform SM2 antibody
      see all

    图片

    • Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567) at 1/5000 dilution (purified) + Human testis lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 227 kDa
      Observed band size: 227 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

      Immunocytochemistry/ Immunofluorescence analysis of A-673 (Human muscle Ewing's Sarcoma) cells labeling smooth muscle Myosin heavy chain 11 with purified ab133567 at 1/50 dilution (2.34 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling smooth muscle Myosin heavy chain 11 with purified ab133567 at 1/1000 dilution (0.12 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

      Immunohistochemical analysis of paraffin-embedded Human ovary tissue labelling smooth muscle Myosin heavy chain 11 with unpurified ab133567 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      All lanes : Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567) at 1/1000 dilution (unpurified)

      Lane 1 : Human bladder lysate
      Lane 2 : Human artery lysate
      Lane 3 : Human testis lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 227 kDa

    • Flow Cytometry (Intracellular) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Flow Cytometry (Intracellular) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

      Intracellular Flow Cytometry analysis of 293 (Human embryonic kidney epithelial cell) cells labeling smooth muscle Myosin heavy chain 11 (red) with purified ab133567 at a 1/20 dilution (10ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

      Immunohistochemical analysis of paraffin-embedded Human prostate tissue labelling smooth muscle Myosin heavy chain 11 with unpurified ab133567 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)This image is courtesy of an anonymous abreview.

      unpurified ab133567 staining smooth muscle Myosin heavy chain 11 in Human smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized and blocked in 1% serum, 0.1%triton, 0.1% BSA in PBS. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. A Goat anti-rabbit IgG Alexa 488 (green) was used as the secondary antibody, and DAPI was used to stain cell nuclei (blue).

      See Abreview

    • Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)
      Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)] (ab133567)

    文献 (16)

    发表研究结果有使用 ab133567？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab133567 被引用在 16 文献中.

    • Huang MX  et al. Circ_0022920 Maintains the Contractile Phenotype of Human Aortic Vascular Smooth Muscle Cells Via Sponging microRNA-650 and Promoting Transforming Growth Factor Beta Receptor 1 Expression in Angiotensin II-Induced Models for Aortic Dissection. J Am Heart Assoc 12:e027425 (2023). PubMed: 36974747
    • Zhou Y  et al. SMYD2 regulates vascular smooth muscle cell phenotypic switching and intimal hyperplasia via interaction with myocardin. Cell Mol Life Sci 80:264 (2023). PubMed: 37615725
    • Jia S  et al. Pea Peptide Supplementation in Conjunction With Resistance Exercise Promotes Gains in Muscle Mass and Strength. Front Nutr 9:878229 (2022). PubMed: 35873424
    • Seime T  et al. Biomechanical Assessment of Macro-Calcification in Human Carotid Atherosclerosis and Its Impact on Smooth Muscle Cell Phenotype. Cells 11:N/A (2022). PubMed: 36291144
    • Hu Y  et al. Lactate promotes vascular smooth muscle cell switch to a synthetic phenotype by inhibiting miR-23b expression. Korean J Physiol Pharmacol 26:519-530 (2022). PubMed: 36302626
    View all Publications for this product

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5336(B)]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Lung, Skeletal Muscle, Heart and Aorta)
    Gel Running Conditions
    Reduced Denaturing (5% Tris-glycine)
    Loading amount
    10 µg
    Specification
    Lung, Skeletal Muscle, Heart and Aorta
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Rebecca Deaton

    Verified customer

    提交于 Mar 19 2019

    Immunocytochemistry/ Immunofluorescence

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    1%serum,0.1%triton,0.1%BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Sample
    Human Cell (smooth muscle cells)
    Specification
    smooth muscle cells
    Permeabilization
    Yes - see blocking buffer
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 07 2013

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