重组Anti-smooth muscle Myosin heavy chain 11抗体[EPR5335]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (AB124679)

Unpurified ab124679, at 1/100 dilution, staining smooth muscle Myosin heavy chain 11 in paraffin-embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (AB124679)

Immunohistochemical staining of paraffin embedded human smooth muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (AB124679)

Unpurified ab124679, at 1/100 dilution, staining smooth muscle Myosin heavy chain 11 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (AB124679)

Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab124679 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

• WB

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Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (AB124679)

All lanes:

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (ab124679) at 1/10000 dilution

Lane 1:

Human fetal artery lysate at 10 µg

Lane 2:

Human bladder lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 227 kDa

Observed band size: 250 kDa

false

• WB

Lab

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (AB124679)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (ab124679) at 1/50000 dilution

All lanes:

human fetal artery lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 227 kDa

Observed band size: 250 kDa

false

• WB

Lab

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (AB124679)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (ab124679) at 1/20000 dilution

All lanes:

mouse spleen at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 227 kDa

Observed band size: 250 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-smooth muscle Myosin heavy chain 11 antibody [EPR5335] (AB124679)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.