Anti-smooth muscle Myosin heavy chain 11抗体(ab125884)
Key features and details
- Rabbit polyclonal to smooth muscle Myosin heavy chain 11
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-smooth muscle Myosin heavy chain 11抗体
参阅全部 smooth muscle Myosin heavy chain 11 一抗 -
描述
兔多克隆抗体to smooth muscle Myosin heavy chain 11 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Pig, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
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免疫原
Synthetic peptide corresponding to Human smooth muscle Myosin heavy chain 11 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab153753) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125884于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 227 kDa (predicted molecular weight: 227 kDa).
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| IHC-P | (1) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 227 kDa (predicted molecular weight: 227 kDa). |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Muscle contraction. -
组织特异性
Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea. -
疾病相关
Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content. -
序列相似性
Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
结构域
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
细胞定位
Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils. - Information by UniProt
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数据库链接
- Entrez Gene: 4629 Human
- Entrez Gene: 17880 Mouse
- Entrez Gene: 100627924 Pig
- Entrez Gene: 100009145 Rabbit
- Entrez Gene: 24582 Rat
- Omim: 160745 Human
- SwissProt: P35749 Human
- SwissProt: O08638 Mouse
see all -
别名
- AAT4 antibody
- DKFZp686D10126 antibody
- DKFZp686D19237 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody (ab125884)
IHC image of smooth muscle Myosin heavy chain 11 staining in a section of formalin-fixed paraffin-embedded normal human heart* performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab125884, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-smooth muscle Myosin heavy chain 11 antibody (ab125884)Anti-smooth muscle Myosin heavy chain 11 antibody (ab125884) at 1 µg/ml + Bladder (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 227 kDa
Observed band size: 227 kDa
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125884 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab125884 被引用在 14 文献中.
- DeRoo E et al. A vein wall cell atlas of murine venous thrombosis determined by single-cell RNA sequencing. Commun Biol 6:130 (2023). PubMed: 36721040
- Zhu H et al. Identification of the hub genes associated with prostate cancer tumorigenesis. Front Oncol 13:1168772 (2023). PubMed: 37251946
- Hu Y et al. Palmitic acid inhibits vascular smooth muscle cell switch to synthetic phenotype via upregulation of miR-22 expression. Aging (Albany NY) 14:8046-8060 (2022). PubMed: 36227173
- Kalisch-Smith JI et al. Analysis of Placental Arteriovenous Formation Reveals New Insights Into Embryos With Congenital Heart Defects. Front Genet 12:806136 (2021). PubMed: 35126469
- He X et al. Kindlin-2 deficiency induces fatal intestinal obstruction in mice. Theranostics 10:6182-6200 (2020). PubMed: 32483447