Anti-SMG5抗体(ab33033)
Key features and details
- Rabbit polyclonal to SMG5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SMG5抗体 -
描述
兔多克隆抗体to SMG5 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Human SMG5.
参阅Abcam的专有抗源政策 (Peptide available as ab36628.) -
阳性对照
- Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab33033于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 114 kDa).
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| IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 114 kDa). |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
应用说明
Is unsuitable for ICC/IF.
靶标
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相关性
SMG5 plays a role in nonsense mediated mRNA decay. It promotes dephosphorylation of RENT1, and is necessary for TERT activity. It is part of a complex that contains SMG1, SMG5, SMG7, PP2AC, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated RENT1/UPF1. -
细胞定位
Cytoplasmic and Nuclear -
数据库链接
- Entrez Gene: 23381 Human
- Entrez Gene: 229512 Mouse
- Omim: 610962 Human
- SwissProt: Q9UPR3 Human
- SwissProt: Q6ZPY2 Mouse
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别名
- EST1 like protein B antibody
- EST1B antibody
- LPTS interacting protein antibody
see all
图片
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Western blot - Anti-SMG5 antibody (ab33033)Anti-SMG5 antibody (ab33033) at 1/250 dilution +
Jurkat whole cell lysate (ab7899) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?The band seen at 140 kDa is consistent with the banding pattern observed for other commercially available antibodies to SMG5.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMG5 antibody (ab33033)IHC image of ab33033 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33033, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab33033 被引用在 5 文献中.
- Huth M et al. NMD is required for timely cell fate transitions by fine-tuning gene expression and regulating translation. Genes Dev 36:348-367 (2022). PubMed: 35241478
- Park Y et al. Nonsense-mediated mRNA decay factor UPF1 promotes aggresome formation. Nat Commun 11:3106 (2020). PubMed: 32561765
- Kurosaki T et al. NMD-degradome sequencing reveals ribosome-bound intermediates with 3'-end non-templated nucleotides. Nat Struct Mol Biol 25:940-950 (2018). PubMed: 30275517
- Wada M et al. Interplay between coronavirus, a cytoplasmic RNA virus, and nonsense-mediated mRNA decay pathway. Proc Natl Acad Sci U S A 115:E10157-E10166 (2018). PubMed: 30297408
- Park OH et al. Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay. Genes Dev 30:2093-2105 (2016). PubMed: 27798850