Anti-SMEK2抗体(ab70622)
Key features and details
- Rabbit polyclonal to SMEK2
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-SMEK2抗体
参阅全部 SMEK2 一抗 -
描述
兔多克隆抗体to SMEK2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chinese hamster
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免疫原
Synthetic peptide corresponding to Human SMEK2 aa 700-800 (C terminal). Synthetic peptide corresponding to a region between residue 715 and the C-terminus (residue 764) of human SMEK2 (NP_065196.1)
Database link: Q5MIZ7 -
阳性对照
- Whole cell lysates from HeLa, 293T and NIH3T3 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab70622于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
靶标
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相关性
SMEK2 contains a WH1 domain. There are five named isoforms produced by alternative splicing. Mapping of a diet-induced hypercholesterolemia locus (Dihc2) in rats identified SMEK2 as a strong candidate for responsiveness to dietary cholesterol. -
细胞定位
Cytoplasm. Cytoskeleton. Centrosome. Nucleus. Note: In interphase localized in the cytoplasm and (with higher levels) the nucleus. During metaphase located in pericentriolar regions. -
数据库链接
- Entrez Gene: 57223 Human
- Entrez Gene: 104570 Mouse
- Omim: 610352 Human
- SwissProt: Q5MIZ7 Human
- SwissProt: Q922R5 Mouse
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别名
- FLFL2 antibody
- FLJ31474 antibody
- KIAA1387 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMEK2 antibody (ab70622)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SMEK2 with ab70622 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-SMEK2 antibody (ab70622)All lanes : Anti-SMEK2 antibody (ab70622) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 97 kDa
Observed band size: 97 kDa -
Immunoprecipitation - Anti-SMEK2 antibody (ab70622)Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab70622 at 3 µg/ml (lane 1) or control IgG (lane 2). 20% of immunoiprecipitate was analysed by Western blot using ab70622 at 1 µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab70622 被引用在 1 文献中.
- Ho MY et al. PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-?B signaling in lung cancer. Oncotarget 7:52255-52269 (2016). PubMed: 27391343