Anti-SMEK2抗体(ab224222)
Key features and details
- Rabbit polyclonal to SMEK2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-SMEK2抗体
参阅全部 SMEK2 一抗 -
描述
兔多克隆抗体to SMEK2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human SMEK2 aa 650-750. aa729-732 are modified.
Database link: Q5MIZ7 -
阳性对照
- WB: NIH/3T3, NBT-II, RT4, EFO-21 and A431 cell lysates. ICC/IF: U-251 MG cells. IHC-P: Human cerebral cortex, testis and fallopian tube tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab224222于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 97 kDa.
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| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
| 说明 |
|---|
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 97 kDa. |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
靶标
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相关性
SMEK2 contains a WH1 domain. There are five named isoforms produced by alternative splicing. Mapping of a diet-induced hypercholesterolemia locus (Dihc2) in rats identified SMEK2 as a strong candidate for responsiveness to dietary cholesterol. -
细胞定位
Cytoplasm. Cytoskeleton. Centrosome. Nucleus. Note: In interphase localized in the cytoplasm and (with higher levels) the nucleus. During metaphase located in pericentriolar regions. -
数据库链接
- Entrez Gene: 57223 Human
- Entrez Gene: 104570 Mouse
- Entrez Gene: 360993 Rat
- Omim: 610352 Human
- SwissProt: Q5MIZ7 Human
- SwissProt: Q922R5 Mouse
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别名
- FLFL2 antibody
- FLJ31474 antibody
- KIAA1387 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMEK2 antibody (ab224222)
Paraffin-embedded human fallopian tube tissue stained for SMEK2 using ab224222 at 1/50 dilution in immunohistochemical analysis. Shows strong nuclear positivity in glandular cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMEK2 antibody (ab224222)Paraffin-embedded human testis tissue stained for SMEK2 using ab224222 at 1/50 dilution in immunohistochemical analysis. Shows strong nuclear postitivity in seminiferous ducts cells.
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Immunocytochemistry/ Immunofluorescence - Anti-SMEK2 antibody (ab224222)PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for SMEK2 (green) using ab224222 at 4 µg/ml in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMEK2 antibody (ab224222)Paraffin-embedded human cerebral cortex tissue stained for SMEK2 using ab224222 at 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-SMEK2 antibody (ab224222)All lanes : Anti-SMEK2 antibody (ab224222) at 1/100 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : EFO-21 cell lysate
Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 97 kDa -
Western blot - Anti-SMEK2 antibody (ab224222)All lanes : Anti-SMEK2 antibody (ab224222) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Developed using the ECL technique.
Predicted band size: 97 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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