Anti-SMCHD1抗体(ab31865)
Key features and details
- Rabbit polyclonal to SMCHD1
- Suitable for: ICC/IF, WB, ChIP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
-
产品名称
Anti-SMCHD1抗体
参阅全部 SMCHD1 一抗 -
描述
兔多克隆抗体to SMCHD1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, ChIPmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human SMCHD1 aa 1750-1850 conjugated to keyhole limpet haemocyanin.
(Peptide available asab31864) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31865于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | (1) |
1/100.
|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 226 kDa. PubMed: 21836164
|
| ChIP |
Use at an assay dependent concentration. PubMed: 28587678
|
| 说明 |
|---|
|
ICC/IF
1/100. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 226 kDa. PubMed: 21836164 |
|
ChIP
Use at an assay dependent concentration. PubMed: 28587678 |
靶标
- Information by UniProt
-
数据库链接
- Entrez Gene: 23347 Human
- Entrez Gene: 74355 Mouse
- Omim: 614982 Human
- SwissProt: A6NHR9 Human
- SwissProt: Q6P5D8 Mouse
- Unigene: 8118 Human
- Unigene: 194450 Mouse
-
别名
- BAMS antibody
- FSHD2 antibody
- KIAA0650 antibody
see all
图片
-
Immunocytochemistry/ Immunofluorescence - Anti-SMCHD1 antibody (ab31865)This image is courtesy of an Abreview submitted by Dr Kirk McManusab31865 (1/100) staining SMCHD1 by immunofluorescence in assynchronous HeLa cells (green). Secondary antibody: goat polyclonal antibody conjugated to Cy3; Counterstain: DAPI (red). Please refer to Abreview for further information.
-
Immunocytochemistry/ Immunofluorescence - Anti-SMCHD1 antibody (ab31865)ICC/IF image of ab31865 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31865, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methaol fixed (5 min) HeLa, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
数据表及文件
-
SDS download
-
Datasheet download
文献 (20)
ab31865 被引用在 20 文献中.
- Laberthonnière C et al. In skeletal muscle and neural crest cells, SMCHD1 regulates biological pathways relevant for Bosma syndrome and facioscapulohumeral dystrophy phenotype. Nucleic Acids Res 51:7269-7287 (2023). PubMed: 37334829
- Šikrová D et al. SMCHD1 and LRIF1 converge at the FSHD-associated D4Z4 repeat and LRIF1 promoter yet display different modes of action. Commun Biol 6:677 (2023). PubMed: 37380887
- Tapia Del Fierro A et al. SMCHD1 has separable roles in chromatin architecture and gene silencing that could be targeted in disease. Nat Commun 14:5466 (2023). PubMed: 37749075
- Huang Z et al. The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins. Sci Adv 7:N/A (2021). PubMed: 33523915
- Theurillat I et al. Extensive SUMO Modification of Repressive Chromatin Factors Distinguishes Pluripotent from Somatic Cells. Cell Rep 32:108146 (2020). PubMed: 32937131