Anti-SMC5抗体(ab18038)
Key features and details
- Rabbit polyclonal to SMC5
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-SMC5抗体
参阅全部 SMC5 一抗 -
描述
兔多克隆抗体to SMC5 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
-
免疫原
Synthetic peptide within Human SMC5 aa 1050 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8IY18 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Antibody was purified using an epitope specific to SMC5 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab18038于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2-10 µg/mg of lysate.
|
|
| WB |
1/5000 - 1/25000. Detects a band of approximately 145 kDa (predicted molecular weight: 129 kDa).
|
| 说明 |
|---|
|
IP
Use at 2-10 µg/mg of lysate. |
|
WB
1/5000 - 1/25000. Detects a band of approximately 145 kDa (predicted molecular weight: 129 kDa). |
靶标
-
功能
Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome (PubMed:26983541). -
组织特异性
Widely expressed (PubMed:11408570). Strongly expressed in testis (PubMed:11408570). -
序列相似性
Belongs to the SMC family. SMC5 subfamily. -
结构域
The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC6, forming a V-shaped heterodimer. -
翻译后修饰
Sumoylated.
Ubiquitinated.
(Microbial infection) SMC5-SMC6 complex is degraded by the activity of Hepatitis B X protein. -
细胞定位
Nucleus. Chromosome. Nucleus, PML body. Chromosome, telomere. Associates with chromatin (PubMed:25931565). Colocalizes with SMC6 on the X-Y chromosome pair within the sex vesicle during late pachytene/diplotene (By similarity). Localizes to PML nuclear bodies in ALT cell lines (PubMed:17589526). Accumulates with RAD18 and the SLF1-SLF2 complex at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565). - Information by UniProt
-
数据库链接
- Entrez Gene: 23137 Human
- Entrez Gene: 293967 Rat
- Omim: 609386 Human
- SwissProt: Q8IY18 Human
- Unigene: 534189 Human
- Unigene: 601181 Human
-
别名
- hSMC 5 antibody
- hSMC5 antibody
- KIAA0594 antibody
see all
图片
-
Western blot - Anti-SMC5 antibody (ab18038)
Ab18038 staining Human SMC5 at a concentration of 0.1
µ µ Samples:
A) Nuclear extract (10 and 20
µ B) Whole cell lysate (500
µ Detection: Chemiluminescence with exposure times of less than 2 minutes.
Ab18038 staining Human SMC5 at a concentration of 0.1 µg/ml by Western Blot, and 5 µg/0.5 mg lysate by Immunoprecipitation.Samples:
A) Nuclear extract (10 and 20 µg) from HeLa cells.
B) Whole cell lysate (500 µg) from HeLa and 293T cells.
Detection: Chemiluminescence with exposure times of less than 2 minutes.
-
Immunoprecipitation - Anti-SMC5 antibody (ab18038)Immunoprecipitation analysis of whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer.
Lane 1: ab18038 used for IP at 6 µg per reaction
Lane 2: Control IgG
For blotting immunoprecipitated SMC5, ab18038 was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab18038 被引用在 4 文献中.
- Sekiba K et al. Inhibition of HBV Transcription From cccDNA With Nitazoxanide by Targeting the HBx-DDB1 Interaction. Cell Mol Gastroenterol Hepatol 7:297-312 (2019). PubMed: 30704981
- Gallego-Paez LM et al. Smc5/6-mediated regulation of replication progression contributes to chromosome assembly during mitosis in human cells. Mol Biol Cell 25:302-17 (2014). Human . PubMed: 24258023
- Gómez R et al. Dynamic localization of SMC5/6 complex proteins during mammalian meiosis and mitosis suggests functions in distinct chromosome processes. J Cell Sci 126:4239-52 (2013). PubMed: 23843628
- Li J et al. Identification of a novel proliferation-related protein, WHSC1 4a, in human gliomas. Neuro Oncol 10:45-51 (2008). PubMed: 18182627