AB179831

重组Anti-SMC4抗体[EPR13366] - C-terminal

Anti-SMC4 antibody [EPR13366] - C-terminal

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal SMC4 antibody. C-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

查看别名

CAPC, SMC4L1, SMC4, Structural maintenance of chromosomes protein 4, SMC protein 4, SMC-4, Chromosome-associated polypeptide C, XCAP-C homolog, hCAP-C

3 Images

Flow Cytometry (Intracellular) - Anti-SMC4 antibody [EPR13366] - C-terminal (AB179831)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-SMC4 antibody [EPR13366] - C-terminal (AB179831)

Intracellular flow cytometric analysis of permeabilized Raji cells using ab179831 at 1/10 (red) or a rabbit IgG negative (green).

Immunocytochemistry/ Immunofluorescence - Anti-SMC4 antibody [EPR13366] - C-terminal (AB179831)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SMC4 antibody [EPR13366] - C-terminal (AB179831)

Immunofluorescent analysis of HeLa cells labeling SMC4 with ab179831 at 1/50 dilution.

Supplier Data

Western blot - Anti-SMC4 antibody [EPR13366] - C-terminal (AB179831)

All lanes:

Western blot - Anti-SMC4 antibody [EPR13366] - C-terminal (ab179831) at 1/1000 dilution

Lane 1:

Ramos cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

Raji cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 147 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13366

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, Flow Cyt (Intra), WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

style

left-column

性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SMC4 also known as Structural Maintenance of Chromosomes protein 4 is a core component of the condensin complex. It weighs approximately 141 kDa and localizes to the nucleus of cells. SMC4 plays a mechanical role during mitosis where it ensures chromosomes condense correctly allowing for proper segregation. This protein's expression occurs widely across different tissue types reflecting its critical involvement in cell division and genomic integrity.

Biological function summary

SMC4 operates as a part of the condensin complex which consists of several proteins including SMC2. This complex is essential in chromosome architecture mediating the compression and cohesion of sister chromatids. This structural role stabilizes chromosomes to prevent damage during cell cycle phases. By maintaining chromosomal morphology SMC4 contributes to genetic stability across generations of cells.

Pathways

SMC4 functions prominently within the chromosome segregation and cell cycle pathways. Alongside its partner SMC2 it helps manage the chromosomal landscape during mitotic events. This positioning within pathways highlights interactions with other key proteins such as cohesin which also plays a role in chromosome cohesion. By ensuring proper chromosomal condensation SMC4 synchronizes with various checkpoints throughout the cell cycle.

SMC4 has links to certain cancers including glioblastoma and colorectal cancer where abnormal expression or function can disrupt mitosis and promote genomic instability. In these contexts SMC4 often interacts with other cancer-related proteins such as p53 which plays roles in cell cycle regulation and apoptosis. The misregulation of SMC4 can contribute to unchecked cell proliferation and tumor development making it a significant focus of cancer research.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.

See full target information SMC4

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cancers 11: PubMed31357676

2019

Diminished Condensin Gene Expression Drives Chromosome Instability That May Contribute to Colorectal Cancer Pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Allison K Baergen,Lucile M Jeusset,Zelda Lichtensztejn,Kirk J McManus

Biochemical and biophysical research communication 505:1112-1120 PubMed30336977

2018

The proteomic study of serially passaged human skin fibroblast cells uncovers down-regulation of the chromosome condensin complex proteins involved in replicative senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Meng,Jing Gao,Hongwen Zhu,Han He,Zhi Lu,Minhua Hong,Hu Zhou

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-SMC4抗体[EPR13366] - C-terminal

Anti-SMC4 antibody [EPR13366] - C-terminal

Flow Cytometry (Intracellular) - Anti-SMC4 antibody [EPR13366] - C-terminal (AB179831)

Immunocytochemistry/ Immunofluorescence - Anti-SMC4 antibody [EPR13366] - C-terminal (AB179831)

Western blot - Anti-SMC4 antibody [EPR13366] - C-terminal (AB179831)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原