AB9263

Anti-SMC3 抗体

Name: SMC3抗体 (ab9263)| Abcam中文官网
Brand: Abcam Limited
SKU: AB9263
Price: 5453 CNY
Availability: InStock
Rating: 5 (7 reviews)

Anti-SMC3 antibody

(7 Reviews)

(111 Publications)

Anti-SMC3 antibody (ab9263) is a rabbit polyclonal antibody detecting SMC3 in Western Blot, IP, IHC-P. Suitable for Human, Mouse.

- Over 90 publications
- Trusted since 2002

查看别名

BAM, BMH, CSPG6, SMC3L1, SMC3, Structural maintenance of chromosomes protein 3, SMC protein 3, SMC-3, Basement membrane-associated chondroitin proteoglycan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, Bamacan, hCAP

5 Images

Supplier Data

Immunoprecipitation - Anti-SMC3 antibody (AB9263)

Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer.

Lane 1 : ab9263 used for IP at 6 μg per reaction

Lane 2 : Control IgG

For blotting immunoprecipitated SMC3, ab9263 was used at 0.04 μg/mL.

Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-SMC3 antibody (ab9263)

Predicted band size: 141 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (AB9263)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (AB9263)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling SMC3 with ab9263 at 1/5000 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (AB9263)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse renal cell carcinoma tissue labeling SMC3 with ab9263 at 1/5000 dilution. Heat mediated antigen retrieval was performed using citrate buffer pH 6.

Supplier Data

Western blot - Anti-SMC3 antibody (AB9263)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-SMC3 antibody (ab9263) at 0.04 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 10 µg

Lane 4:

LNCaP (human prostate cancer cell line) whole cell lysate at 10 µg

Lane 5:

U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 10 µg

Lane 6:

A549 (human lung carcinoma cell line) whole cell lysate at 10 µg

Lane 7:

TCMK-1 (mouse epithelial cell line from kidney transformed with SV40 viral antigen) whole cell lysate at 10 µg

Lane 8:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

Predicted band size: 141 kDa

false

Exposure time: 3s

CiteAb

Western blot - Anti-SMC3 antibody (AB9263)

Western Blotting using Anti-SMC3 antibody, ab9263. Publication image from Kadota, S. et al., 2020, Nat Commun, 32451376. Legend direct from paper.

NUP153 interacts with CTCF and cohesin.a Silver stain showing proteins that immunoprecipitated (IP) with FLAG-NUP153. b Co-IP shows FLAG-NUP153 interaction with CTCF, and cohesin subunits, SMC3, SMC1A, and RAD21. NUP153 was pulled down using anti-FLAG antibody. c Schematic showing steps of chromatin fractionation assay in HeLa cells. d NUP153 was detected in the nuclear insoluble fraction (P2) along with CTCF and cohesin. NUP153 detected in the chromatin-associated soluble fraction (S3) following micrococcal nuclease (MNase) treatment of P1 fraction. Ppt, precipitate; Sup, supernatant; Nucleoporin 62, NUP62; loading controls : α-TUBULIN (cytoplasm), Histone H3 (chromatin). Experiments were repeated twice. Source data are provided as a Source Data file.

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IHC-P, WB, IP

applications

免疫原

Synthetic Peptide within Human SMC3 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9UQE7

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/25000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-SMC3 antibody (ab9263) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of SMC3?
Anti-SMC3 (ab9263) specifically detects a band for SMC3 (UniProt: Q9UQE7) at a molecular weight of 141.5kDa.

Trusted by the scientific community
Anti-SMC3 (ab9263) was first used in a scientific publication in 2002 and has been cited over 90 times in peer-reviewed journals.

Reviewed by scientists
Anti-SMC3 (ab9263) has over 5 independent reviews from customers.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nmof 1.4 equals 1.0 mg of IgG.

存储溶液

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SMC3 also known as Structural Maintenance of Chromosomes 3 is a core component of the cohesin complex playing a vital role in chromosomal architecture and stability. It has a molecular mass of approximately 141 kDa. SMC3 is ubiquitously expressed across various tissues and is notably abundant in proliferating cells. Using an ATPase domain SMC3 helps in the ATP-dependent binding and release of cohesin from chromatin contributing to sister chromatid cohesion during cell division.

Biological function summary

Cohesin complexes rely on SMC3 for the maintenance of chromosome cohesion condensation and repair. The protein is essential for the proper segregation of chromosomes during mitosis and meiosis. SMC3 part of the cohesin complex along with SMC1 RAD21 and SCC3 forms a ring-like structure encircling sister chromatids ensuring they remain attached until anaphase. Besides structural purposes SMC3 facilitates DNA damage response contributing to genome integrity by allowing homologous recombination.

Pathways

SMC3 is integral in the cell cycle regulation and DNA repair pathways. During the cell cycle SMC3 cooperates with SMC1 and RAD21 in the establishment of cohesin networks necessary for genome integrity. In terms of DNA repair SMC3 supports homologous recombination an important mechanism of DNA double-strand break repair. The protein interacts with PLK1 and CDK1 influencing progression through the G2/M checkpoint in response to DNA damage.

SMC3 is linked with Cornelia de Lange syndrome a developmental disorder characterized by growth and cognitive delays. Mutations in SMC3 disrupt its ability to maintain chromosome cohesion leading to defects in DNA replication and repair processes. Additionally SMC3 aberrations associate with cohesinopathies a group of diseases related to mutations in cohesin complex components like NIPBL and SMC1 highlighting SMC3's importance in genetic stability and normal development.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex also plays an important role in spindle pole assembly during mitosis and in chromosomes movement.

See full target information SMC3

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文献 (111)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40806572

2025

Ectopic Recruitment of the CTCF N-Terminal Domain with Two Proximal Zinc-Finger Domains as a Tool for 3D Genome Engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Eugenia A Tiukacheva,Artem V Luzhin,Natalia Kruglova,Anastasia S Shtompel,Grigorii Antonov,Anna Tvorogova,Yegor Vassetzky,Sergey V Ulianov,Sergey V Razin

Current biology : CB 35:3943-3958.e5 PubMed40730158

2025

Cohesin-mediated stabilization of the CCAN complex at kinetochores in mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Julian Haase,Koly Aktar,Mary Kate Bonner,Leonard Colin,Hindol Gupta,Briana E Marinoni,David O Morgan,Alexander E Kelly

Epigenetics & chromatin 18:33 PubMed40490791

2025

Cohesin stabilization at promoters and enhancers by common transcription factors and chromatin regulators.

Applications

Unspecified application

Species

Unspecified reactive species

Audra F Bryan,Megan Justice,Alexis V Stutzman,Daniel J McKay,Jill M Dowen

Epigenetics & chromatin 18:31 PubMed40452075

2025

Unraveling the cohesin-chromatin interface: identifying protein interactions that modulate chromosome structure and function.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie L Rittenhouse,Riya Gohil,June E Arricastres,Jill M Dowen

Science (New York, N.Y.) 388:eadq1709 PubMed40208986

2025

Rules of engagement for condensins and cohesins guide mitotic chromosome formation.

Applications

Unspecified application

Species

Unspecified reactive species

Kumiko Samejima,Johan H Gibcus,Sameer Abraham,Fernanda Cisneros-Soberanis,Itaru Samejima,Alison J Beckett,Nina Pučeková,Maria Alba Abad,Christos Spanos,Bethan Medina-Pritchard,James R Paulson,Linfeng Xie,A Arockia Jeyaprakash,Ian A Prior,Leonid A Mirny,Job Dekker,Anton Goloborodko,William C Earnshaw

PLoS pathogens 21:e1012467 PubMed40198713

2025

A targeted CRISPR screen identifies ETS1 as a regulator of HIV-1 latency.

Applications

Unspecified application

Species

Unspecified reactive species

Manickam Ashokkumar,Terry L Hafer,Abby Felton,Nancie M Archin,David M Margolis,Michael Emerman,Edward P Browne

Scientific reports 15:9918 PubMed40121293

2025

Cohesin and condensin regulate chromosome topology and play an essential role in maintaining pluripotency in embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eui-Hwan Choi,Keun P Kim

Cell genomics 5:100813 PubMed40118069

2025

Binding domain mutations provide insight into CTCF's relationship with chromatin and its contribution to gene regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Catherine Do,Guimei Jiang,Giulia Cova,Christos C Katsifis,Domenic N Narducci,Theodore Sakellaropoulos,Raphael Vidal,Priscillia Lhoumaud,Aristotelis Tsirigos,Faye Fara D Regis,Nata Kakabadze,Elphege P Nora,Marcus Noyes,Anders S Hansen,Jane A Skok

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e70181 PubMed39545685

2024

TAD-dependent sub-TAD is required for enhancer-promoter interaction enabling the β-globin transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Dasoul Lee,Jin Kang,AeRi Kim

Cytogenetic and genome research 164:1-15 PubMed38452741

2024

Spaghetti Connections: Synaptonemal Complexes as a Tool to Explore Chromosome Structure, Evolution, and Meiotic Behavior in Fish.

Applications

Unspecified application

Species

Unspecified reactive species

Artem Lisachov,Dmitrij Dedukh,Sergey Simanovsky,Thitipong Panthum,Worapong Singchat,Kornsorn Srikulnath

View all publications

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Anti-SMC3 抗体

Anti-SMC3 antibody

Immunoprecipitation - Anti-SMC3 antibody (AB9263)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (AB9263)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC3 antibody (AB9263)

Western blot - Anti-SMC3 antibody (AB9263)

Western blot - Anti-SMC3 antibody (AB9263)