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Cell Biology Cell Cycle Cell Division Chromatid Cohesion

Anti-SMC1A (phospho S966)抗体(ab1276)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (17)

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Western blot - Anti-SMC1A (phospho S966) antibody (ab1276)
  • Immunoprecipitation - Anti-SMC1A (phospho S966) antibody (ab1276)
  • Immunocytochemistry - Anti-SMC1A (phospho S966) antibody (ab1276)
  • Immunocytochemistry - Anti-SMC1A (phospho S966) antibody (ab1276)

Key features and details

  • Rabbit polyclonal to SMC1A (phospho S966)
  • Suitable for: ICC, IP, WB
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-SMC1A (phospho S966)抗体
    参阅全部 SMC1A 一抗
  • 描述

    兔多克隆抗体to SMC1A (phospho S966)
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC, IP, WBmore details
  • 种属反应性

    与反应: Human
    预测可用于: Rhesus monkey, Orangutan
  • 免疫原

    Synthetic peptide corresponding to SMC1A (phospho S966) conjugated to keyhole limpet haemocyanin.
    Database link: Q14683

  • 阳性对照

    • WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate. ICC: HeLa and SKN cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 纯化说明

    Antibodies that were not phospho-specific were removed by solid phase absorption. Antibodies specific for SMC1A pSer957 were affinity purified using the phosphopeptide immobilized onsolid support.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.
    • Epigenetics and Nuclear Signaling
    • Chromosome Structure
    • Cohesins
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab1276于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC (1)
1/500 - 1/5000.
IP
Use at 2-5 µg/mg of lysate.
WB (1)
1/500 - 1/5000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
说明
ICC
1/500 - 1/5000.
IP
Use at 2-5 µg/mg of lysate.
WB
1/500 - 1/5000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).

靶标

  • 功能

    Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.
  • 疾病相关

    Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies.
  • 序列相似性

    Belongs to the SMC family. SMC1 subfamily.
  • 结构域

    The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure.
  • 翻译后修饰

    Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation.
  • 细胞定位

    Nucleus. Chromosome. Chromosome > centromere > kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis.
  • Target information above from: UniProt accession Q14683 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 8243 Human
    • Omim: 300040 Human
    • SwissProt: Q14683 Human
    • Unigene: 211602 Human
    • 别名

      • Chromosome segregation protein SmcB antibody
      • DXS423E antibody
      • KIAA0178 antibody
      • MGC138332 antibody
      • Sb1.8 antibody
      • Segregation of mitotic chromosomes 1 antibody
      • SMC protein 1A antibody
      • SMC-1-alpha antibody
      • SMC-1A antibody
      • SMC1 (structural maintenance of chromosomes 1 yeast) like 1 antibody
      • SMC1 antibody
      • SMC1 structural maintenance of chromosomes 1 like 1 antibody
      • SMC1A antibody
      • SMC1A_HUMAN antibody
      • SMC1alpha antibody
      • SMC1L1 antibody
      • SMCB antibody
      • Structural maintenance of chromosomes 1A antibody
      • Structural maintenance of chromosomes protein 1A antibody
      see all

    图片

    • Western blot - Anti-SMC1A (phospho S966) antibody (ab1276)
      Western blot - Anti-SMC1A (phospho S966) antibody (ab1276)
      All lanes : Anti-SMC1A (phospho S966) antibody (ab1276) at 0.1 µg/ml

      Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with 100 µM etoposide (+)
      Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with 100 µM etoposide (-)

      Lysates/proteins at 50 µg per lane.

      Predicted band size: 143 kDa


      Exposure time: 3 minutes
    • Immunoprecipitation - Anti-SMC1A (phospho S966) antibody (ab1276)
      Immunoprecipitation - Anti-SMC1A (phospho S966) antibody (ab1276)

      SMC1A was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate treated with 100 µM etoposide (+) or mock treated (-) with ab1276 at 1 µg/ml. Western blot was performed from the immunoprecipitate using ab1276 at 1/1000 dilution.

       

    • Immunocytochemistry - Anti-SMC1A (phospho S966) antibody (ab1276)
      Immunocytochemistry - Anti-SMC1A (phospho S966) antibody (ab1276)

      Immunocytochemistry analysis of neocarzinostatin treated asynchronous HeLa cells labelling SMC1A (phospho S966) with ab1276 at 1/5000 (0.2µg/ml). A DyLight® 594-conjugated anti-rabbit IgG (1/100) was used as the secondary antibody.

    • Immunocytochemistry - Anti-SMC1A (phospho S966) antibody (ab1276)
      Immunocytochemistry - Anti-SMC1A (phospho S966) antibody (ab1276)This image was submitted as part of a review by Kirk McManus, University of British Columbia

      ICC of SKN cells cultured on coverslips were fixed in 4% paraformaldehyde and then stained with Rabbit polyclonal to SMC1A (phospho S966), ab1276 (green) at a working dilution of 1/200. The DNA stained with DAPI is shown in red. (100x magnification).

      See Abreview

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (17)

    发表研究结果有使用 ab1276?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab1276 被引用在 17 文献中.

    • Yi F  et al. The deacetylation-phosphorylation regulation of SIRT2-SMC1A axis as a mechanism of antimitotic catastrophe in early tumorigenesis. Sci Adv 7:N/A (2021). PubMed: 33627431
    • Leimbacher PA  et al. MDC1 Interacts with TOPBP1 to Maintain Chromosomal Stability during Mitosis. Mol Cell 74:571-583.e8 (2019). PubMed: 30898438
    • Zhang Y  et al. Phosphorylation of SMC1A promotes hepatocellular carcinoma cell proliferation and migration. Int J Biol Sci 14:1081-1089 (2018). PubMed: 29988990
    • Yang T  et al. Quercetin-3-O-ß-D-glucoside decreases the bioavailability of cyclosporin A through regulation of drug metabolizing enzymes, transporters and nuclear receptors in rats. Mol Med Rep 18:2599-2612 (2018). PubMed: 30015887
    • Jiang Y  et al. ATM function and its relationship with ATM gene mutations in chronic lymphocytic leukemia with the recurrent deletion (11q22.3-23.2). Blood Cancer J 6:e465 (2016). PubMed: 27588518
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Western blot abreview for Anti-SMC1 (phospho S966) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cells)
    Loading amount
    40 µg
    Specification
    HeLa cells
    Treatment
    X radiation (10g)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Mar 28 2007

    Immunocytochemistry abreview for Anti-SMC1 (phospho S966) antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry
    Sample
    Human Cell (HeLa and SK-N-SH)
    Specification
    HeLa and SK-N-SH
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Kirk Mcmanus

    Verified customer

    提交于 Oct 12 2005

    Question

    Is this antibody for SMC1A or SMC1B? The Database links are SMC1B. But unlike SMC1A, there is no S966 or S957 sites in SMC1B.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 06 2010

    Answer

    Thank you for your enquiry. I can confirm that the immunogen used to generate ab1276 SMC1 (phospho S966) antibody represents a portion of human Structural Maintenance of Chromosomes 1 (GeneID 8243) around serine 966 according to the numbering given in entry NP_006297.2. The sequence NP_006297.2 represents SMC 1A. An alignment of SMC1A and SMC1B indicates that the two isoforms do not bear similar epitopes in this region; therefore I would suggest that ab1276 recognizes S966 in the SMC1A protein. I hope this would be helpful to you. Should you have any further quesitons, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    回复于 Aug 06 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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