Anti-SMAP2抗体(ab121675)
Key features and details
- Rabbit polyclonal to SMAP2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-SMAP2抗体
参阅全部 SMAP2 一抗 -
描述
兔多克隆抗体to SMAP2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
antigen sequence, corresponding to C terminal amino acids 326-429 of Human SMAP2.
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阳性对照
- Human lymph node tissue; A431 cells; RT 4, U 251 MG, Human Liver and Tonsil cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121675于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
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功能
GTPase activating protein that acts on ARF1. Can also activate ARF6 (in vitro). May play a role in clathrin-dependent retrograde transport from early endosomes to the trans-Golgi network. -
序列相似性
Contains 1 Arf-GAP domain. -
细胞定位
Cytoplasm. Detected in multiple foci throughout the cytoplasm and in juxtanuclear structures. - Information by UniProt
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数据库链接
- Entrez Gene: 64744 Human
- Entrez Gene: 69780 Mouse
- Entrez Gene: 298500 Rat
- SwissProt: Q8WU79 Human
- SwissProt: Q7TN29 Mouse
- Unigene: 15200 Human
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别名
- RP1-228H13.3 antibody
- Small ArfGAP2 antibody
- SMAP 1L antibody
see all
图片
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Western blot - Anti-SMAP2 antibody (ab121675)Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) -
Western blot - Anti-SMAP2 antibody (ab121675)All lanes : Anti-SMAP2 antibody (ab121675) at 1/250 dilution
Lane 1 : RT 4 lysate
Lane 2 : U 251 MG lysate
Lane 3 : Human plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human tonsil lysate
Developed using the ECL technique. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAP2 antibody (ab121675)ab121675 at 1/300 dilution staining SMAP2 in Paraffin-embedded Human lymph node tissue by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-SMAP2 antibody (ab121675)ab121675 at 4 µg/ml staining SMAP2 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121675 尚未被引用在任何文献中。