Anti-Smad2 + Smad3抗体(ab63672)
Key features and details
- Rabbit polyclonal to Smad2 + Smad3
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Smad2 + Smad3抗体
参阅全部 Smad2 + Smad3 一抗 -
描述
兔多克隆抗体to Smad2 + Smad3 -
宿主
Rabbit -
特异性
ab63672 detects endogenous levels of total Smad2 and Smad3. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human, African green monkey
预测可用于: Mouse
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免疫原
Synthetic non-phosphopeptide derived from human Smad2 and Smad3 around the phosphorylation site of threonine 8 (P-F-TP-P-P).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab63672于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 48 kDa.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 48 kDa. |
靶标
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相关性
SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. -
细胞定位
Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. -
数据库链接
- Entrez Gene: 4087 Human
- Entrez Gene: 4088 Human
- Entrez Gene: 17126 Mouse
- Entrez Gene: 17127 Mouse
- Omim: 601366 Human
- Omim: 603109 Human
- SwissProt: P84022 Human
- SwissProt: Q15796 Human
see all -
别名
- hMAD 2 antibody
- hMAD 3 antibody
- hSMAD2 antibody
see all
图片
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Western blot - Anti-Smad2 + Smad3 antibody (ab63672)All lanes : Anti-Smad2 + Smad3 antibody (ab63672) at 1/500 dilution
Lane 1 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts treated with UV (15mins)
Lane 2 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts untreated
Lane 3 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins)
Lane 4 : Jurkat cell extracts treated with EGF (200ng/ml, 30mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Observed band size: 48 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab63672 被引用在 17 文献中.
- Liao X et al. LncRNA-Gm9866 promotes liver fibrosis by activating TGFβ/Smad signaling via targeting Fam98b. J Transl Med 21:778 (2023). PubMed: 37919785
- Yamamoto A et al. Therapeutic Effect of Cyclin-Dependent Kinase 4/6 Inhibitor on Dermal Fibrosis in Murine Models of Systemic Sclerosis. Arthritis Rheumatol 74:860-870 (2022). PubMed: 34882985
- Liao CG et al. Active demethylation upregulates CD147 expression promoting non-small cell lung cancer invasion and metastasis. Oncogene 41:1780-1794 (2022). PubMed: 35132181
- Yin J et al. Isoflurane Postconditioning Upregulates Phosphorylated Connexin 43 in the Middle Cerebral Artery Occlusion Model and Is Probably Associated with the TGF-ß1/Smad2/3 Signaling Pathway. Biomed Res Int 2020:3451215 (2020). PubMed: 32258113
- Zong M et al. Irbesartan ameliorates myocardial fibrosis in diabetic cardiomyopathy rats by inhibiting the TGFß1/Smad2/3 pathway. Exp Ther Med 20:117 (2020). PubMed: 33005243