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Anti-SMAD1/5/9抗体(ab80255)

Reviews (3)Q&A (2)References (13)
Western blot - Anti-SMAD1/5/9 antibody (ab80255)

    Key features and details

    • Rabbit polyclonal to SMAD1/5/9
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    选择批间可重复性更高的重组抗体

    Product image
    Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-SMAD1/5/9抗体
      参阅全部 SMAD1/5/9 一抗

    • 描述

      兔多克隆抗体to SMAD1/5/9

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human
      预测可用于: Mouse

    • 免疫原

      Synthetic peptide derived from N terminal of human SMAD1/5/9

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab80255于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (2)
    1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
    说明
    WB
    1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

    靶标

    • 相关性

      SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs, common-mediator SMAD and inhibitory SMADs. SMAD1, SMAD5 and SMAD9 have high degrees of homology. SMAD8 and SMAD9 are used as alternate names for the same protein. Human SMAD1 is a 465 amino acid protein, human SMAD5 is a 465 amino acid and human SMAD9 is a 430 amino acid protein.

    • 细胞定位

      Cytoplasmic and Nuclear

    • 数据库链接

      see all

    • 别名

      • AI528653 antibody
      • BSP1 antibody
      • DKFZp781C1895 antibody
      • DKFZp781O1323 antibody
      • Dwarfin A antibody
      • Dwf A antibody
      • Dwfc antibody
      • hSMAD1 antibody
      • hSmad5 antibody
      • JV4 1 antibody
      • JV41 antibody
      • JV5 1 antibody
      • MAD (mothers against decapentaplegic, Drosophila) homolog 1 antibody
      • MAD (mothers against decapentaplegic, Drosophila) homolog 9 antibody
      • MAD homolog 1 antibody
      • MAD homolog 9 antibody
      • MAD homolog1 (mothers against decapentaplegic, Drosophila) antibody
      • Mad related protein 1 antibody
      • MAD, mothers against decapentaplegic homolog 9 antibody
      • Madh1 antibody
      • MADH5 antibody
      • MADH6 antibody
      • MADH9 antibody
      • MADR1 antibody
      • Mlp1 antibody
      • Mothers against decapentaplegic, drosophila, homolog of, 9 antibody
      • Mothers against DPP homolog 1 antibody
      • Mothers against DPP homolog 9 antibody
      • MusMLP antibody
      • OTTHUMP00000178565 antibody
      • OTTMUSP00000022970 antibody
      • OTTMUSP00000022971 antibody
      • OTTMUSP00000022972 antibody
      • OTTMUSP0000002324 antibody
      • OTTMUSP00000023428 antibody
      • Sma- and Mad-related protein 1 antibody
      • SMAD antibody
      • SMAD family member 5 antibody
      • SMAD8 antibody
      • SMAD8A antibody
      • SMAD8B antibody
      • TGF beta signaling protein 1 antibody
      • Transforming growth factor-beta signaling protein 1 antibody
      see all

    图片

    • Western blot - Anti-SMAD1/5/9 antibody (ab80255)
      Western blot - Anti-SMAD1/5/9 antibody (ab80255)
      All lanes : Anti-SMAD1/5/9 antibody (ab80255) at 1/500 dilution

      Lane 1 : HeLa cell extracts
      Lane 2 : HeLa cell extracts with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 52 kDa
      Observed band size: 52 kDa

    文献 (13)

    发表研究结果有使用 ab80255？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab80255 被引用在 13 文献中.

    • Chen H  et al. Secreted phosphoprotein 24 kD (Spp24) inhibits the growth of human osteosarcoma through the BMP-2/Smad signaling pathway. J Orthop Res 41:1803-1814 (2023). PubMed: 36883270
    • Kabwe JC  et al. CRISPR-mediated Bmpr2 point mutation exacerbates late pulmonary vasculopathy and reduces survival in rats with experimental pulmonary hypertension. Respir Res 23:87 (2022). PubMed: 35395852
    • Kim JH  et al. Albiflorin Promotes Osteoblast Differentiation and Healing of Rat Femoral Fractures Through Enhancing BMP-2/Smad and Wnt/β-Catenin Signaling. Front Pharmacol 12:690113 (2021). PubMed: 34349649
    • Nakajima T  et al. Changes in Smad1/5/9 expression and phosphorylation in astrocytes of the rat hippocampus after transient global cerebral ischemia. J Chem Neuroanat 113:101941 (2021). PubMed: 33711423
    • Jin T  et al. CHI3L1 promotes Staphylococcus aureus-induced osteomyelitis by activating p38/MAPK and Smad signaling pathways. Exp Cell Res 403:112596 (2021). PubMed: 33826950
    View all Publications for this product

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-SMAD1/5/9 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (chondrocytes)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    chondrocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jun 12 2018

    Western blot abreview for Anti-SMAD1/5/9 antibody

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (embryonic stem cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    embryonic stem cells
    Blocking step
    Odyssey blocking buffer, LiCor as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 01 2016

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