Anti-Smad1 (phospho S206)抗体(ab106092)
Key features and details
- Rabbit polyclonal to Smad1 (phospho S206)
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Smad1 (phospho S206)抗体
参阅全部 Smad1 一抗 -
描述
兔多克隆抗体to Smad1 (phospho S206) -
宿主
Rabbit -
特异性
This affinity-purified antibody is directed against the phosphorylated form of human SMAD1 protein at the pS206 residue. Reactivity occurs against human SMAD1 pS206 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human SMAD1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest 100% cross reactivity with SMAD1 from human, Duckbill platypus, Drosophila, mouse, opossum, chimpanzee, bonobo, orangutan, gorilla, macaque, cattle, sheep, pig, rat, horse, salmon, chicken, and dog based on the sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
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经测试应用
适用于: WB, ELISAmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Sheep, Chicken, Cow, Dog, Pig, Chimpanzee, Drosophila melanogaster, Macaque monkey, Gorilla, Opossum, Orangutan
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免疫原
Synthetic peptide corresponding to Human Smad1 (phospho S206).
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阳性对照
- HEPG2 and HaCaT whole cell lysates, treated with BMP2, EGF, or NaCl.
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常规说明
Expiration date is one year from date of opening. Dilute only prior to immediate use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
This affinity-purified antibody is directed against the phosphorylated form of human SMAD1 protein at the pS206 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106092于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500.
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| ELISA |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
1/500. |
|
ELISA
Use at an assay dependent concentration. |
靶标
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功能
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
组织特异性
Ubiquitous. Highest expression seen in the heart and skeletal muscle. -
序列相似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻译后修饰
Phosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 540488 Cow
- Entrez Gene: 4086 Human
- Entrez Gene: 17125 Mouse
- Omim: 601595 Human
- SwissProt: Q1JQA2 Cow
- SwissProt: Q15797 Human
- SwissProt: P70340 Mouse
- Unigene: 604588 Human
see all -
别名
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab106092 尚未被引用在任何文献中。