Anti-Smad1抗体(ab131550)
Key features and details
- Rabbit polyclonal to Smad1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Smad1抗体
参阅全部 Smad1 一抗 -
描述
兔多克隆抗体to Smad1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide conjugated to KLH, corresponding to a region around C terminal amino acids 461-465 (
ISSVS
) of Human Smad1 (NP_001003688.1). -
阳性对照
- HeLa cells; HeLa cell extract
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131550于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa).
|
|
ICC/IF |
1/100 - 1/200.
|
说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 52 kDa). |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
组织特异性
Ubiquitous. Highest expression seen in the heart and skeletal muscle. -
序列相似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻译后修饰
Phosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4086 Human
- Omim: 601595 Human
- SwissProt: Q15797 Human
- Unigene: 604588 Human
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别名
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
see all
图片
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Immunofluorescent analysis of methanol-fixed HeLa cells labelling Smad1 with ab131550 at 1/100 dilution.
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Anti-Smad1 antibody (ab131550) at 1/500 dilution + HeLa cell extract
Predicted band size: 52 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab131550 被引用在 1 文献中.
- Li J et al. TRAF4 positively regulates the osteogenic differentiation of mesenchymal stem cells by acting as an E3 ubiquitin ligase to degrade Smurf2. Cell Death Differ 26:2652-2666 (2019). PubMed: 31076633