Anti-Smad1抗体[913C1b] (ab53745)
![Flow Cytometry - Anti-Smad1 antibody [913C1b] (ab53745)](https://www.abcam.cn/ps/products/53/ab53745/Images/ab53745-66598-anti-smad1-antibody-913c1b-flow-cytometry.jpg "Flow Cytometry - Anti-Smad1 antibody [913C1b] (ab53745)")
Key features and details
- Mouse monoclonal [913C1b] to Smad1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-Smad1抗体[913C1b]
参阅全部 Smad1 一抗 -
描述
小鼠单克隆抗体[913C1b] to Smad1 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cytmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment corresponding to Human Smad1.
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阳性对照
- WB: HEK293, F2408 and NIH3T3 whole cell lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
存储溶液
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
913C1b -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53745于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/50. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa).
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/50. Detects a band of approximately 60 kDa (predicted molecular weight: 52 kDa). |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. -
组织特异性
Ubiquitous. Highest expression seen in the heart and skeletal muscle. -
序列相似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻译后修饰
Phosphorylated on serine by BMP type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4086 Human
- Entrez Gene: 17125 Mouse
- Entrez Gene: 25671 Rat
- Omim: 601595 Human
- SwissProt: Q15797 Human
- SwissProt: P70340 Mouse
- SwissProt: P97588 Rat
- Unigene: 604588 Human
see all -
别名
- BSP-1 antibody
- BSP1 antibody
- HsMAD1 antibody
see all
图片
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Flow Cytometry - Anti-Smad1 antibody [913C1b] (ab53745)Using 0.5µg Anti-SMAD1 antibody (ab53745) and 0.25µg Goat anti-Mouse secondary.
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![Western blot - Anti-Smad1 antibody [913C1b] (ab53745)](https://www.abcam.cn/ps/products/53/ab53745/Images/ab53745-66597-anti-smad1-antibody-913c1b-western-blot.jpg)
Western blot - Anti-Smad1 antibody [913C1b] (ab53745)Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + HEK293 whole cell lysate at 25 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-Smad1 antibody [913C1b] (ab53745)](https://www.abcam.cn/ps/products/53/ab53745/Images/ab53745-66596-anti-smad1-antibody-913c1b-western-blot.jpg)
Western blot - Anti-Smad1 antibody [913C1b] (ab53745)Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + NIH3T3 whole cell lysate at 25 µg
Secondary
Mouse IgG at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-Smad1 antibody [913C1b] (ab53745)](https://www.abcam.cn/ps/products/53/ab53745/Images/ab53745-66595-anti-smad1-antibody-913c1b-western-blot.jpg)
Western blot - Anti-Smad1 antibody [913C1b] (ab53745)Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution + F2408 whole cell lysate at 25 µg
Secondary
Mouse IgG at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (3)
ab53745 被引用在 3 文献中.
- Mendez PL et al. Atheroprone fluid shear stress-regulated ALK1-Endoglin-SMAD signaling originates from early endosomes. BMC Biol 20:210 (2022). PubMed: 36171573
- Zhang D et al. MiR-144-5p, an exosomal miRNA from bone marrow-derived macrophage in type 2 diabetes, impairs bone fracture healing via targeting Smad1. J Nanobiotechnology 19:226 (2021). PubMed: 34340698
- Caja L et al. Snail regulates BMP and TGFß pathways to control the differentiation status of glioma-initiating cells. Oncogene 37:2515-2531 (2018). PubMed: 29449696
