重组Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467)抗体[EP728(2)AY] (ab76296)
![Western blot - Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] (ab76296)](https://www.abcam.cn/ps/products/76/ab76296/Images/ab76296-477145-anti-smad5-phospho-s463-s465-antibody-western-blot-c6-rat.jpg "Western blot - Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] (ab76296)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP728(2)AY] to Smad1 (phospho S463 + S465) + SMAD5 (phospho S463 + S465) + SMAD9 (phospho S465 + S467)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467)抗体[EP728(2)AY] -
描述
兔单克隆抗体[EP728(2)AY] to Smad1 (phospho S463 + S465) + SMAD5 (phospho S463 + S465) + SMAD9 (phospho S465 + S467) -
宿主
Rabbit -
特异性
Detects SMAD5 phosphorylated at Serine 463 and 465. This antibody may cross-react with Smad1 Phospho (pS463/465) and Smad9 Phospho (pS465/467).
Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.
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经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa cytoplasmic or nuclear lysate treated with BMP-4
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EP728(2)AY -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76296于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500. Predicted molecular weight: 52 kDa.
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| 说明 |
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WB
1/500. Predicted molecular weight: 52 kDa. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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细胞定位
Smad1: Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. SMAD5: Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. SMAD9: Cytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4. -
数据库链接
- Entrez Gene: 4086 Human
- Entrez Gene: 4090 Human
- Entrez Gene: 4093 Human
- Entrez Gene: 17125 Mouse
- Entrez Gene: 17129 Mouse
- Entrez Gene: 55994 Mouse
- Entrez Gene: 25671 Rat
- Entrez Gene: 59328 Rat
see all
图片
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Western blot - Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] (ab76296)All lanes : Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] (ab76296) at 1/1000 dilution
Lane 1 : Untreated C6 (Rat glial tumor glial cell) whole cell lysate with NFDM/TBST
Lane 2 : C6 treated with 10ng/ml TGF beta 3 and 50µM MG-132 for 30 min whole cell lysate with NFDM/TBST
Lane 3 : C6 treated with 10ng/ml TGF beta 3 and 50µM MG-132 for 30 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour with NFDM/TBST
Lysates/proteins at 15 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 52 kDa
Exposure time: 7 seconds -
![Western blot - Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] (ab76296)](https://www.abcam.cn/ps/products/76/ab76296/Images/ab76296-477141-anti-smad5-phospho-s463-s465-antibody-western-blot-hela-human.jpg)
Western blot - Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] (ab76296)All lanes : Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] (ab76296) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate with NFDM/TBST
Lane 2 : HeLa treated with 20ng/ml TGF beta 1 for 15 min whole cell lysate with NFDM/TBST
Lane 3 : HeLa treated with 20ng/ml TGF beta 1 for 15 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour with NFDM/TBST
Lysates/proteins at 15 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 52 kDa
Exposure time: 20 seconds -
![Western blot - Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] (ab76296)](https://www.abcam.cn/ps/products/76/ab76296/Images/ab76296-477142-anti-smad5-phospho-s463-s465-antibody-western-blot-nih-3t3-mouse.jpg)
Western blot - Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] (ab76296)All lanes : Anti-SRGAP1 antibody (ab76926) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 treated with 20ng/ml TGF beta 1 and 50µM MG-132 for 15 min whole cell lysate
Lane 3 : NIH/3T3 treated with 20ng/ml TGF beta 1 and 50µM MG-132 for 15 min whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 100 secondsBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] (ab76296)](https://www.abcam.cn/ps/products/76/ab76296/Images/ab76296-91671-anti-smad5-phospho-s463-s465-antibody-ep7282ay-western-blot.jpg)
Western blot - Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] (ab76296)All lanes : Anti-SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467) antibody [EP728(2)AY] (ab76296) at 1/500 dilution
Lane 1 : HeLa cell lysate - untreated
Lane 2 : HeLa cell lysate - treated with BMP-4
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
Anti-SMAD1 (phospho S463+465)+ SMAD5 (phospho S463+465)+ SMAD9 (phospho S465+S467) (ab76296)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab76296 被引用在 5 文献中.
- Zhou Q et al. A New Insight of Kartogenin Induced the Mesenchymal Stem Cells (MSCs) Selectively Differentiate into Chondrocytes by Activating the Bone Morphogenetic Protein 7 (BMP-7)/Smad5 Pathway. Med Sci Monit 25:4960-4967 (2019). PubMed: 31271564
- Cui Y et al. Single-Cell Transcriptome Analysis Maps the Developmental Track of the Human Heart. Cell Rep 26:1934-1950.e5 (2019). PubMed: 30759401
- Peng J et al. The BMP signaling pathway leads to enhanced proliferation in serous ovarian cancer-A potential therapeutic target. Mol Carcinog 55:335-45 (2016). PubMed: 25663289
- Ghiabi P et al. Breast cancer cells promote a notch-dependent mesenchymal phenotype in endothelial cells participating to a pro-tumoral niche. J Transl Med 13:27 (2015). WB ; Human . PubMed: 25623554
- Nili M et al. Soluble Repulsive Guidance Molecule c/Hemojuvelin Is a Broad Spectrum Bone Morphogenetic Protein (BMP) Antagonist and Inhibits both BMP2- and BMP6-mediated Signaling and Gene Expression. J Biol Chem 285:24783-92 (2010). WB ; Human . PubMed: 20530805