AB108965

重组Anti-SMAD 1 + SMAD9抗体[EPR2290(2)]

Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)]

RabMAb
Recombinant
了解详情

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(5 Publications)

Rabbit Recombinant Monoclonal SMAD1 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

查看别名

BSP1, MADH1, MADR1, SMAD1, Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, Transforming growth factor-beta-signaling protein 1, SMAD 1, Smad1, hSMAD1, BSP-1

1 Images

Unknown

Western blot - Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (AB108965)

All lanes:

Western blot - Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (ab108965) at 1/1000 dilution

Lane 1:

293T lysate at 10 µg

Lane 2:

A673 lysate at 10 µg

Lane 3:

Human heart lysate at 10 µg

Lane 4:

HeLa lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 58 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR2290(2)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

SMAD1 and SMAD9 also known as Mad1 and MadH1 are members of the SMAD family of proteins. These proteins are essential components of the BMP (Bone Morphogenetic Protein) signaling pathway. SMAD1 has a molecular weight of approximately 52 kDa while SMAD9 is slightly smaller. Both proteins are expressed in various tissues throughout the body including bone heart and brain where they contribute to cellular processes by transducing signals from the cell membrane to the nucleus.

Biological function summary

SMAD1 and SMAD9 mediate transcriptional responses to BMP signals influencing gene expression that governs cell growth differentiation and embryonic development. They function as part of a complex typically forming heterodimers with SMAD4. Upon BMP receptor activation SMAD1 and SMAD9 become phosphorylated which allows them to interact with SMAD4 and translocate to the nucleus to regulate target gene expression.

Pathways

SMAD1 and SMAD9 are key in the BMP signaling pathway and interact closely with SMAD4. The BMP pathway plays a critical role in developmental processes such as osteogenesis and chondrogenesis. SMAD1 and SMAD9 are also involved with the TGF-beta signaling pathway showing interaction with proteins like SMAD2 and SMAD3 through cross-talk which adds complexity to the regulatory circuitry for cell fate decisions.

SMAD1 and SMAD9 are associated with skeletal disorders and cancer. Mutations in the BMP pathway involving SMAD1 and SMAD9 can contribute to bone-related diseases such as fibrodysplasia ossificans progressiva. Their aberrant signaling is also linked to cancer development with disruptions in BMP signaling pathways potentially leading to tumor progression. These proteins are connected to other molecules like Noggin and Chordin which are involved in modulating BMP activity in these disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis (PubMed : 9335504). Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor (PubMed : 33667543). In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network (PubMed : 33667543). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Positively regulates BMP4-induced expression of odontogenic development regulator MSX1 following IPO7-mediated nuclear import (By similarity).

See full target information SMAD1

其他靶点

SMAD9

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

NPJ Regenerative medicine 10:26 PubMed40461558

2025

Engineered BMP2/BMP7 extracellular vesicles induce autocrine BMP release driving SMAD phosphorylation to promote bone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Zeji Du,Skylar A Rizzo,Tiffany L Sarrafian,Monique S Bagwell,Ryan C Mahlberg,Ashley Amontree,Paige Schiebel,Dinah M Tauferner,Zoe S LeBrasseur,Tyra A Witt,Mary Nagel,Kyla A Boyd,Humberto De Vitto,Matthew L Hillestad,Paul G Stalboerger,Matthew T Houdek,Rafael J Sierra,Atta Behfar

Analytical cellular pathology (Amsterdam) 2024:6624283 PubMed39624161

2024

N-Acetylcysteine Treats Spinal Cord Injury by Inhibiting Astrocyte Proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Zhang,Chaoxi Qin,Fei Meng,Xiaopeng Han,Xing Guo

Cancer research 84:3761-3771 PubMed39207369

2024

Live-Cell Invasive Phenotyping Uncovers ALK2 as a Therapeutic Target in LKB1-Mutant Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junghui Koo,Chang-Soo Seong,Rebecca E Parker,Amy Herrera,Bhakti Dwivedi,Robert A Arthur,Ashok Reddy Dinasarapu,Henry Richard Johnston,Henry Claussen,Carol Tucker-Burden,Suresh S Ramalingam,Haian Fu,Wei Zhou,Adam I Marcus,Melissa Gilbert-Ross

Acta biochimica et biophysica Sinica 52:1337-1347 PubMed33313721

2020

LncRNA HOTAIR promotes endometrial fibrosis by activating TGF-β1/Smad pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhong Wu,Lingge Jin,Yudi Zhang,Aihong Duan,Juhong Liu,Ziwen Jiang,Liang Huang,Jing Chen,Zhaohui Liu,Dan Lu,Yinmei Dai

American journal of physiology. Endocrinology and metabolism 313:E12-E25 PubMed28351832

2017

Gonad-related factors promote muscle performance gain during postnatal development in male and female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Ueberschlag-Pitiot,Amalia Stantzou,Julien Messéant,Megane Lemaitre,Daniel J Owens,Philippe Noirez,Pauline Roy,Onnik Agbulut,Daniel Metzger,Arnaud Ferry

View all publications

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重组Anti-SMAD 1 + SMAD9抗体[EPR2290(2)]