Anti-Smac/Diablo抗体[SMAC 17 1-87] (ab111893)

Immunohistochemistry (paraffin-treated sections) analysis of human colon tissue labelling SMAC/Diablo with ab111893 at 1/100 dilution. Tissues were counterstained with hematoxylin and dehydrated with xylene and ethanol. Negative control (left) has no primary antibody. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (paraffin-treated sections) analysis of mouse testis tissue labelling SMAC/Diablo with ab111893 at 1/100 dilution. Tissues were counterstained with hematoxylin and dehydrated with xylene and ethanol. Negative control (left) has no primary antibody. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (paraffin-treated sections) analysis of human testis tissue labelling SMAC/Diablo with ab111893 at 1/100 dilution. Tissues were counterstained with hematoxylin and dehydrated with xylene and ethanol. Negative control (left) has no primary antibody. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

ab111893 labelling Smac / Diablo (green) in the cytoplasm of A431 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

ab111893 labelling Smac / Diablo (green) in the cytoplasm of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

ab111893 labelling Smac / Diablo (green) in the cytoplasm of PC12 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.