Anti-Smac/Diablo抗体(ab8115)
Key features and details
- Rabbit polyclonal to Smac/Diablo
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Smac/Diablo抗体
参阅全部 Smac/Diablo 一抗 -
描述
兔多克隆抗体to Smac/Diablo -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat -
免疫原
Peptide (
SDEGADQEEEAYLRED
)corresponding to amino acids 222 to 237 of murineSmac/DIABLO (2). -
阳性对照
- WB: Mouse and rat heart tissue lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. |
靶标
-
功能
Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). -
组织特异性
Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. -
结构域
The mature N-terminus mediates interaction with XIAP. -
细胞定位
Mitochondrion. Released into the cytosol when cells undergo apoptosis. - Information by UniProt
-
数据库链接
- Entrez Gene: 66593 Mouse
- Entrez Gene: 288753 Rat
- SwissProt: Q9JIQ3 Mouse
- Unigene: 46716 Mouse
-
别名
- 0610041G12Rik antibody
- DBLOH_HUMAN antibody
- DBOH antibody
see all
图片
-
Western blot - Anti-Smac/Diablo antibody (ab8115)All lanes : Anti-Smac/Diablo antibody (ab8115) at 1 µg/ml
Lane 1 : Mouse heart tissue lysate with absence of blocking peptide
Lane 2 : Mouse heart tissue lysate with presence of blocking peptide
Lane 3 : Rat heart tissue lysate with absence of blocking peptide
Observed band size: 25 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
-
Datasheet download
文献 (7)
ab8115 被引用在 7 文献中.
- Takahashi J et al. Interleukin-22 Directly Activates Myocardial STAT3 (Signal Transducer and Activator of Transcription-3) Signaling Pathway and Prevents Myocardial Ischemia Reperfusion Injury. J Am Heart Assoc 9:e014814 (2020). PubMed: 32301368
- Wischhof L et al. A disease-associated Aifm1 variant induces severe myopathy in knockin mice. Mol Metab N/A:N/A (2018). PubMed: 29780003
- Liu S et al. Silencing Livin improved the sensitivity of colon cancer cells to 5-fluorouracil by regulating crosstalk between apoptosis and autophagy. Oncol Lett 15:7707-7715 (2018). PubMed: 29740490
- Shephard F et al. Analysis of Mitochondrial haemoglobin in Parkinson's disease brain. Mitochondrion 29:45-52 (2016). WB . PubMed: 27181046
- Admoni-Elisha L et al. Novel Biomarker Proteins in Chronic Lymphocytic Leukemia: Impact on Diagnosis, Prognosis and Treatment. PLoS One 11:e0148500 (2016). Human . PubMed: 27078856
- Shephard F et al. A mitochondrial location for haemoglobins--dynamic distribution in ageing and Parkinson's disease. Mitochondrion 14:64-72 (2014). WB ; Mouse . PubMed: 24333691
- Heazell AE et al. Altered expression of regulators of caspase activity within trophoblast of normal pregnancies and pregnancies complicated by preeclampsia. Reprod Sci 15:1034-43 (2008). WB ; Human . PubMed: 19088373
